Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil
| Autor: | Laurent Poirel, Luisa Zanolli Moreno, Richard Chaby, Nicolas Kieffer, Andrea Micke Moreno, Patrice Nordmann, Aude Breton, Pierre Tissieres |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Swine 030106 microbiology Transferases (Other Substituted Phosphate Groups) Microbial Sensitivity Tests medicine.disease_cause Genetic analysis Microbiology 03 medical and health sciences Plasmid Mechanisms of Resistance Molecular genetics medicine Escherichia coli Transferase Animals Pharmacology (medical) Gene Pharmacology chemistry.chemical_classification biology Colistin Escherichia coli Proteins Genetic Variation biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents Infectious Diseases Enzyme chemistry Brazil Genome Bacterial hormones hormone substitutes and hormone antagonists Plasmids |
| Popis: | A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C 2 plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase. |
| Databáze: | OpenAIRE |
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