Microbial production of uracil by an isolated Methylobacterium sp. WJ4 using methanol
| Autor: | Han Bin Oh, Yuhyun Kwon, Jinwon Lee, Byung-Keun Oh, Sangwoo Kim, Soohyun Park, Insu Song, Wangjun Lee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bioengineering Raw material Applied Microbiology and Biotechnology Biochemistry Biotechnological process 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Republic of Korea heterocyclic compounds Uracil Phylogeny Soil Microbiology biology Chemistry Methanol biology.organism_classification Combinatorial chemistry Carbon Methylobacterium sp Kinetics RNA Bacterial Methylobacterium 030104 developmental biology Genes Bacterial Metabolic Networks and Pathways Bacteria Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. 111:63-66 |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/j.enzmictec.2017.10.003 |
| Popis: | In this study, we report the production of uracil from methanol by an isolated methylotrophic bacterium, Methylobacterium sp. WJ4. The use of methanol as alternative carbon feedstock is attractive option in biotechnology. As a feedstock of biotechnological processes, methanol has distinct advantages over methane. This is not only due to physical and chemical considerations, but also to the properties of the pertinent organisms. Besides, with a wide array of biological activities and synthetic accessibility, uracil is considered as privileged structures in drug discovery. Uracil analogues have been applied to treatments of patients with cancer or viral infections. In this respect, it is meaningful to produce uracil using methanol. The effect of process parameters and methanol concentration for uracil production were investigated and optimized. Uracil production was remarkably increased to 5.76mgg cell dry weight-1 in optimized condition. The results were significant for further understanding of methylotrophic bacteria on uracil production. |
| Databáze: | OpenAIRE |
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