Differential Suppression of Background Mammalian Lysosomal β-Galactosidase Increases the Detection Sensitivity of LacZ-Marked Leukemic Cells
| Autor: | A. C. M. Martens, P. J. Hendrikx, A. Hagenbeek, J. W. M. Visser |
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| Rok vydání: | 1994 |
| Předmět: |
Genetic Markers
Cell Biophysics lac operon Endogeny In Vitro Techniques Biology medicine.disease_cause Sensitivity and Specificity Biochemistry Substrate Specificity chemistry.chemical_compound Rats Inbred BN Escherichia coli medicine Animals Beta-galactosidase Molecular Biology Hymecromone Galactose Galactosides Cell Biology Hydrogen-Ion Concentration beta-Galactosidase Molecular biology Rats Disease Models Animal Kinetics Leukemia Myeloid Acute medicine.anatomical_structure chemistry biology.protein Bone marrow Lysosomes Ex vivo |
| Zdroj: | Analytical Biochemistry. 222:456-460 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.1994.1516 |
| Popis: | A method is described for the detection of Escherichia coli beta-galactosidase-expressing leukemic cells in ex vivo bone marrow samples. 4-Methylumbelliferyl-beta-D-galactopyranoside is used as a substrate in a kinetic assay. D-Galactose is used to suppress endogenous lysosomal beta-galactosidase activity, yielding a sixfold increase in sensitivity. With this assay, the detection limit is one leukemic cell per 10(4) normal bone marrow cells. |
| Databáze: | OpenAIRE |
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