Rice ORMDL controls sphingolipid homeostasis affecting fertility resulting from abnormal pollen development
| Autor: | Johnathan A. Napier, Keasinee Pitnjam, Numphet Sangarwut, Louise V. Michaelson, Sriprapai Chakhonkaen, Malinee Suksangpanomrung, Chutharat Chueasiri, Ketsuwan Chunthong, Kanidta Sangsawang, Amorntip Muangprom |
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| Rok vydání: | 2012 |
| Předmět: |
Candidate gene
Agricultural Biotechnology lcsh:Medicine Plant Science Biochemistry RNA interference Gene Expression Regulation Plant Gene expression Homeostasis lcsh:Science Phylogeny Regulation of gene expression Genetics Multidisciplinary Plant Biochemistry Reproduction food and beverages Agriculture Orosomucoid Genomics Plants Genetically Modified Lipids Pollen RNA Interference Research Article Biotechnology Crops Biology Ceramides Genes Plant Research and Analysis Methods Model Organisms Plant and Algal Models Gene Sphingolipids Gene Expression Profiling lcsh:R Biology and Life Sciences Oryza Genetically modified rice Sphingolipid Biosynthetic Pathways Gene expression profiling Mutation lcsh:Q Rice Crop Science Cereal Crops Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 9, p e106386 (2014) |
| ISSN: | 1932-6203 |
| Popis: | The orosomucoids (ORM) are ER-resisdent polypeptides encoded by ORM and ORMDL (ORM-like) genes. In humans, ORMDL3 was reported as genetic risk factor associated to asthma. In yeast, ORM proteins act as negative regulators of sphingolipid synthesis. Sphingolipids are important molecules regulating several processes including stress responses and apoptosis. However, the function of ORM/ORMDL genes in plants has not yet been reported. Previously, we found that temperature sensitive genetic male sterility (TGMS) rice lines controlled by tms2 contain a deletion of about 70 kb in chromosome 7. We identified four genes expressed in panicles, including an ORMDL ortholog, as candidates for tms2. In this report, we quantified expression of the only two candidate genes normally expressed in anthers of wild type plants grown in controlled growth rooms for fertile and sterile conditions. We found that only the ORMDL gene (LOC_Os07g26940) showed differential expression under these conditions. To better understand the function of rice ORMDL genes, we generated RNAi transgenic rice plants suppressing either LOC_Os07g26940, or all three ORMDL genes present in rice. We found that the RNAi transgenic plants with low expression of either LOC_Os07g26940 alone or all three ORMDL genes were sterile, having abnormal pollen morphology and staining. In addition, we found that both sphingolipid metabolism and expression of genes involved in sphingolipid synthesis were perturbed in the tms2 mutant, analogous to the role of ORMs in yeast. Our results indicated that plant ORMDL proteins influence sphingolipid homeostasis, and deletion of this gene affected fertility resulting from abnormal pollen development. |
| Databáze: | OpenAIRE |
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