Sucrose Represses the Developmentally Controlled Transient Activation of the Plastocyanin Gene in Arabidopsis thaliana Seedlings

Autor:	Patricia Kock, Peter Weisbeek, Paul P. Dijkwel, Sjef Smeekens, Rex Bezemer
Rok vydání:	1996
Předmět:	Messenger RNA biology Physiology Transgene Plant Science biology.organism_classification Cell biology Fusion gene Biochemistry Gene expression Etiolation Genetics Arabidopsis thaliana Luciferase Plastocyanin Research Article
Zdroj:	ResearcherID
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.110.2.455
Popis:	The plastocyanin (PC) gene of Arabidopsis thaliana is activated independently of light during early seedling development. In etiolated seedlings, PC mRNA levels increase transiently and a maximum dark level is reached after 2 d of growth in darkness. In etiolated transgenic seedlings carrying a chimeric PC-promoter: luciferase fusion gene, luciferase activity is similarly increased after 2 d of growth. The transient increase in PC mRNA and luciferase activity levels can be repressed by sucrose. Nonmetabolizable sugars and polyethylene glycol do not have a major effect on PC gene expression. Also, light-grown seedlings show a similar transient and sucrose-sensitive increase in PC mRNA levels and luciferase activity, as in dark-grown seedlings, but here expression levels are 15- fold higher. These findings suggest the presence of a sucrose-sensitive, developmentally controlled expression mechanism that operates independently of light.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7905f3fe5ef5d1e530166dff171ed1c https://doi.org/10.1104/pp.110.2.455 Zobrazit plný text záznamu