Enumeration of interleukin-10-positive B cells from peripheral blood of patients with chronic lymphocytic leukemia
| Autor: | Marco Rossi, Ernesto Vigna, Nadia Caruso, Teresa Granata, Laura De Stefano, Anna Grazia Recchia, Massimo Gentile, Fortunato Morabito, Pierfrancesco Tassone, Pierosandro Tagliaferri, Mariavaleria Pellicanò, Sabrina Bossio, Rosa Toscano |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Chronic lymphocytic leukemia B-Lymphocyte Subsets chemical and pharmacologic phenomena Hematology Biology medicine.disease medicine.disease_cause Leukemia Lymphocytic Chronic B-Cell CD19 Autoimmunity Interleukin-10 Interleukin 10 medicine.anatomical_structure Immune system Immunophenotyping Oncology immune system diseases hemic and lymphatic diseases Immunology medicine biology.protein Humans CD5 B cell |
| Zdroj: | Leukemialymphoma. 55(6) |
| ISSN: | 1029-2403 |
| Popis: | Chronic lymphocytic leukemia (CLL) disease is characterized by generalized immune system dysregulation leading to increased susceptibility to infections and secondary malignancies. Immune suppression is mainly ascribed to the increased number of blood regulatory T-cells and elevated serum levels of interleukin-10 (IL-10), associated with the aberrant activity of circulating dendritic cells [1 – 3]. However, several fi ndings have shown that a specifi c B cell subtype, B-reg, is able to exert immunosuppressive activity. B-regs were fi rst described in mouse models of autoimmunity such as experimental allergic encephalomyelitis, infl ammatory bowel diseases and collagen-induced arthritis [4,5]. Mouse B-reg cells share a common CD19 /CD5 /CD1d phenotype, resembling an immature or primarily mature B-cell, which secretes IL-10 and transforming growth factor- β (TGF- β ), through which most immune suppressive eff ects can be exerted. In humans, emerging data demonstrate that a B-cell subset with B-reg functions exist in both healthy and autoimmune settings [6,7]. Human B-regs represent around 4% of circulating blood B cells and can be distinguished into proB and B10 types [8]. Th e peculiar immune phenotype and functions have raised the question whether this subset may be represented in patients with CLL. Indeed, DiLillo et al . [8] have shown that CD5 CLL B cells share phenotypical similarities with human B-regs and secrete higher amounts of IL-10 upon in vitro stimulation as compared to age-matched healthy controls. However, identifi cation and enumeration of this putative B-reg subset based on stimulation in vitro are not feasible for routine clinical practice. Based on these premises, we addressed whether a B cell subset with a B-reg-like immunophenotype could be identifi ed in the context of CLL B-cell clones by multiparametric fl ow cytometry only. We collected peripheral blood from 24 newly diagnosed patients with stage A CLL and 12 age-matched healthy subjects. Patients with stage A CLL were selected because this is the most frequent presentation of CLL disease at diagnosis. Furthermore, according to DiLillo data, IL-10-producing CLL clones are more often observed in early disease stages as compared to advanced stages [8]. |
| Databáze: | OpenAIRE |
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