Development of novel nucleic acid-loaded Bubble liposomes using cholesterol-conjugated siRNA

Autor:	Yoko Endo-Takahashi, Kazumi Ishii, Kazuo Maruyama, Ryo Suzuki, Yukihiko Aramaki, Yukiko Oguri, Yoichi Negishi, Takashi Murakami
Rok vydání:	2011
Předmět:	Biodistribution Time Factors education Pharmaceutical Science Transfection Ribonucleases hemic and lymphatic diseases PEG ratio Chlorocebus aethiops Gene silencing Animals Tissue Distribution Gene Silencing RNA Small Interfering Ultrasonography Liposome Nuclease Microbubbles biology Chemistry technology industry and agriculture Molecular biology Cholesterol COS Cells Liposomes biology.protein Biophysics Nucleic acid lipids (amino acids peptides and proteins)
Zdroj:	Journal of drug targeting. 19(9)
ISSN:	1029-2330
Popis:	Recently, we developed polyethyleneglycol (PEG)-modified liposomes (Bubble liposomes; BLs) entrapping ultrasound (US) gas and reported that the combination of BLs and US exposure was an effective tool for the delivery of siRNA directly into cells and US-exposed tissues within a short period; however, the results were obtained using a mixture of BLs and naked siRNA. With systemic injections, it is important to control the biodistribution of both BLs and siRNA. In addition, the delivery of siRNA is affected by nuclease degradation and rapid removal from the circulation after intravenous administration. In this study, we attempted to prepare novel siRNA-loaded BLs (chol-si-BLs) using cholesterol-conjugated siRNA (chol-siRNA). We demonstrated that chol-siRNA could be loaded into BLs, leading to the stability of siRNA even in the presence of an RNase. The specific gene-silencing effect was also achieved by transfection with chol-si-BLs and US. Thus, the combination of chol-si-BLs with US exposure is expected to deliver siRNA into a specific tissue via systemic injection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f780cbaa42e2e6acd0529fe7ef6d7d42 https://pubmed.ncbi.nlm.nih.gov/21663557 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.