MT1 and MT2 melatonin receptors are expressed in nonoverlapping neuronal populations

Autor: Paul Pévet, Marie-Paule Felder-Schmittbuhl, Beatrice Guardiola, David Hicks, Paul Klosen, Carole Schuster, Sarawut Lapmanee
Přispěvatelé: Département de Neurobiologie des Rythmes, Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Pôle d'Innovation Thérapeutique Métabolisme, Recherche de Découvertes [Suresnes], Institut de Recherches Internationales Servier [Suresnes] (IRIS), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Pineal Research
Journal of Pineal Research, Wiley, 2019, pp.e12575. ⟨10.1111/jpi.12575⟩
Journal of Pineal Research, Wiley, 2019, 67 (1), pp.e12575. ⟨10.1111/jpi.12575⟩
ISSN: 0742-3098
1600-079X
DOI: 10.1111/jpi.12575⟩
Popis: International audience; Melatonin (MLT) exerts its physiological effects principally through two high-affinity membrane receptors MT1 and MT2. Understanding the exact mechanism of MLT action necessitates the use of highly selective agonists/antagonists to stimulate/inhibit a given MLT receptor. The respective distribution of MT1 and MT2 within the CNS and elsewhere is controversial, and here we used a "knock-in" strategy replacing MT1 or MT2 coding sequences with a LacZ reporter. The data show striking differences in the distribution of MT1 and MT2 receptors in the mouse brain: whereas the MT1 subtype was expressed in very few structures (notably including the suprachiasmatic nucleus and pars tuberalis), MT2 subtype receptors were identified within numerous brain regions including the olfactory bulb, forebrain, hippocampus, amygdala and superior colliculus. Co-expression of the two subtypes was observed in very few structures, and even within these areas they were rarely present in the same individual cell. In conclusion, the expression and distribution of MT2 receptors are much more widespread than previously thought, and there is virtually no correspondence between MT1 and MT2 cellular expression. The precise phenotyping of cells/neurons containing MT1 or MT2 receptor subtypes opens new perspectives for the characterization of links between MLT brain targets, MLT actions and specific MLT receptor subtypes.
Databáze: OpenAIRE
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