The apple MdCOP1-interacting protein 1 negatively regulates hypocotyl elongation and anthocyanin biosynthesis
| Autor: | Chun-Xiang You, Hui Kang, Xiao-Fei Wang, Yu-Jin Hao, Yuxin Yao, Lu-Lu Fu, Tingting Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Crops
Agricultural Anthocyanin Mutant Arabidopsis Repressor Plant Science Genes Plant Hypocotyl Anthocyanins Plant Growth Regulators Gene Expression Regulation Plant lcsh:Botany Gene Plant Proteins CIP1 biology fungi COP1 Apple food and beverages biology.organism_classification Plants Genetically Modified Cell biology lcsh:QK1-989 Transformation (genetics) Malus Photomorphogenesis Ectopic expression Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 21, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1471-2229 |
| Popis: | BackgroundIn plants, CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is a key negative regulator in photoperiod response. However, the biological function of COP1-interacting protein 1 (CIP1) and the regulatory mechanism of the CIP1-COP1 interaction are not fully understood.ResultsHere, we identified the appleMdCIP1gene based on the ArabidopsisAtCIP1gene. Expression pattern analysis showed thatMdCIP1was constitutively expressed in various tissues of apple, and responded to stress and hormone signals at the transcriptional level. Ectopic expression ofMdCIP1complemented the phenotypes of the Arabidopsiscip1mutant, and MdCIP1 inhibited anthocyanin biosynthesis in apple calli. In addition, the biochemical assay demonstrated that MdCIP1 could interact with MdCOP1 protein by their coiled-coil domain, andMdCIP1-OX/cop1–4had a similar phenotype in photomorphogenesis with thecop1–4mutant, suggesting thatCOP1is epistatic toCIP1. Furthermore, the transient transformation assay indicated that MdCIP1 repressed anthocyanin biosynthesis in an MdCOP1-mediated pathway.ConclusionTake together, this study finds that MdCIP1 acts as a repressor in regulating hypocotyl elongation and anthocyanin biosynthesis through MdCOP1 in apple. |
| Databáze: | OpenAIRE |
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