Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region

Autor: Dionicia Gamboa, Renzo Gutierrez-Loli, Katherine Garro, Sandra Morales Ruiz, Jorge Bendezu, Elizabeth Villasis, Pamela Rodriguez, Katherine Torres, Berónica Infante, Manolo Fernández-Díaz
Rok vydání: 2019
Předmět:
purl.org/pe-repo/ocde/ford#3.03.07 [https]
Protozoan Proteins
merozoite surface protein 10
confocal microscopy
Immunoglobulin G
Western blotting
Serology
immunoglobulin G
antibody detection
0302 clinical medicine
Seroepidemiologic Studies
Peru
parasite development
Malaria
Falciparum
Merozoite surface protein
0303 health sciences
biology
medicine.diagnostic_test
protozoal protein
Plasmodium vivax malaria
Amazonas (Brazil)
unclassified drug
3. Good health
Infectious Diseases
purl.org/pe-repo/ocde/ford#3.02.27 [http]
Population Surveillance
Antibody
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
medicine.drug_class
Plasmodium falciparum
030231 tropical medicine
Antigens
Protozoan
purl.org/pe-repo/ocde/ford#3.03.08 [https]
Monoclonal antibody
Article
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Western blot
Antigen
parasitic diseases
medicine
Humans
controlled study
lcsh:RC109-216
protein expression
030304 developmental biology
carboxy terminal sequence
nonhuman
Research
biology.organism_classification
Virology
enzyme linked immunosorbent assay
biology.protein
Monoclonal antibodies
Parasitology
PfMSP10
Peptides
Zdroj: Malaria Journal
Malaria Journal, Vol 18, Iss 1, Pp 1-13 (2019)
CONCYTEC-Institucional
Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica
instacron:CONCYTEC
ISSN: 1475-2875
Popis: Background Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates. Methods IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays. Results Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion. Conclusions The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas.
Databáze: OpenAIRE
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