Sendai virus-based liposomes enable targeted cytosolic delivery of nanoparticles in brain tumor-derived cells

Autor: Veronica Rotari, Maribel Vazquez, Veronica Dudu
Rok vydání: 2012
Předmět:
lcsh:Medical technology
lcsh:Biotechnology
EGFR
Cell
Biomedical Engineering
Brain tumor
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
02 engineering and technology
Stem cell marker
Sendai virus
Applied Microbiology and Biotechnology
03 medical and health sciences
lcsh:TP248.13-248.65
Tumor Cells
Cultured
medicine
cancer
Humans
Progenitor cell
030304 developmental biology
0303 health sciences
Liposome
biology
Quantum dots
Brain Neoplasms
Research
021001 nanoscience & nanotechnology
biology.organism_classification
medicine.disease
Immunohistochemistry
Molecular biology
3. Good health
ErbB Receptors
Virus-based liposomes
medicine.anatomical_structure
lcsh:R855-855.5
Microscopy
Fluorescence
Liposomes
Cancer cell
Cancer research
Nanoparticles
Molecular Medicine
Glioblastoma
0210 nano-technology
Intracellular
Medulloblastoma
Zdroj: Journal of Nanobiotechnology
Journal of Nanobiotechnology, Vol 10, Iss 1, p 9 (2012)
ISSN: 1477-3155
Popis: Background Nanotechnology-based bioassays that detect the presence and/or absence of a combination of cell markers are increasingly used to identify stem or progenitor cells, assess cell heterogeneity, and evaluate tumor malignancy and/or chemoresistance. Delivery methods that enable nanoparticles to rapidly detect emerging, intracellular markers within cell clusters of biopsies will greatly aid in tumor characterization, analysis of functional state and development of treatment regimens. Results Experiments utilized the Sendai virus to achieve in vitro, cytosolic delivery of Quantum dots in cells cultured from Human brain tumors. Using fluorescence microscopy and Transmission Electron Microscopy, in vitro experiments illustrated that these virus-based liposomes decreased the amount of non-specifically endocytosed nanoparticles by 50% in the Human glioblastoma and medulloblastoma samples studied. Significantly, virus-based liposome delivery also facilitated targeted binding of Quantum dots to cytosolic Epidermal Growth Factor Receptor within cultured cells, focal to the early detection and characterization of malignant brain tumors. Conclusions These findings are the first to utilize the Sendai virus to achieve cytosolic, targeted intracellular binding of Qdots within Human brain tumor cells. The results are significant to the continued applicability of nanoparticles used for the molecular labeling of cancer cells to determine tumor heterogeneity, grade, and chemotherapeutic resistivity.
Databáze: OpenAIRE
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