Synthesis of Sonicated Fibrin Nanoparticles that Modulate Fibrin Clot Polymerization and Enhance Angiogenic Responses
| Autor: | Kimberly Nellenbach, Michael A. Daniele, Ashlyn T. Young, Frances S. Ligler, Colleen A. Roosa, Ashley C. Brown, Ismaeel Muhamed |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
02 engineering and technology
Fibrin Tissue Adhesive Fibrinogen 01 natural sciences Fibrin Article Polymerization chemistry.chemical_compound Colloid and Surface Chemistry Thrombin In vivo 0103 physical sciences medicine Physical and Theoretical Chemistry Wound Healing 010304 chemical physics biology Cell growth Cell migration Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology chemistry Biophysics biology.protein Nanoparticles Keratinocyte growth factor 0210 nano-technology Wound healing Biotechnology medicine.drug |
| Zdroj: | Colloids Surf B Biointerfaces |
| Popis: | Chronic wounds can occur when the healing process is disrupted and the wound remains in a prolonged inflammatory stage that leads to severe tissue damage and poor healing outcomes. Clinically used treatments, such as high density, FDA-approved fibrin sealants, do not provide an optimal environment for native cell proliferation and subsequent tissue regeneration. Therefore, new treatments outside the confines of these conventional fibrin bulk gel therapies are required. We have previously developed flowable, low-density fibrin nanoparticles that, when coupled to keratinocyte growth factor, promote cell migration and epithelial wound closure in vivo. Here, we report a new high throughput method for generating the fibrin nanoparticles using probe sonication, which is less time intensive than the previously reported microfluidic method, and investigate the ability of the sonicated fibrin nanoparticles (SFBN) to promote clot formation and cell migration in vitro. The SFBNs can form a fibrin gel when combined with fibrinogen in the absence of exogenous thrombin, and the polymerization rate and fiber density in these fibrin clots is tunable based on SFBN concentration. Furthermore, fibrin gels made with SFBNs support cell migration in an in vitro angiogenic sprouting assay, which is relevant for wound healing. In this report, we show that SFBNs may be a promising wound healing therapy that can be easily produced and delivered in a flowable formulation. |
| Databáze: | OpenAIRE |
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