Arginase‐II promotes melanoma migration and adhesion through enhancing hydrogen peroxide production and STAT3 signaling
Autor: | Yuyan Xiong, Kayluz Frias Boligan, Diogo Ladeiras, Yi Yu, Stephan von Gunten, Xiu-Fen Ming, Zhihong Yang, Robert E. Hunger, Xiujie Liang |
---|---|
Rok vydání: | 2020 |
Předmět: |
STAT3 Transcription Factor
0301 basic medicine Programmed cell death Physiology Clinical Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Cell Adhesion medicine Animals Humans Enzyme Inhibitors STAT3 Melanoma Cell Proliferation Arginase biology Chemistry Cell growth Endothelial Cells Cell migration Hydrogen Peroxide Cell Biology medicine.disease Gene Expression Regulation Neoplastic Endothelial stem cell 030104 developmental biology Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Signal Transduction |
Zdroj: | Journal of Cellular Physiology. 235:9997-10011 |
ISSN: | 1097-4652 0021-9541 |
Popis: | Elevated arginase type II (Arg-II) associates with higher grade tumors. Its function and underlying molecular mechanisms in melanoma remain elusive. In the present study, we observed a significantly higher frequency of Arg-II expression in melanoma of patients with metastasis than those without metastasis. Silencing Arg-II in two human melanoma cell lines slowed down the cell growth, while overexpression of native but not a catalytically inactive Arg-II promoted cell proliferation without affecting cell death. Treatment of cells with arginase inhibitor also reduced melanoma cell number, demonstrating that Arg-II promotes melanoma cell proliferation dependently of its enzymatic activity. However, results from silencing Arg-II or overexpressing native or the inactive Arg-II as well as treatment with arginase inhibitor showed that Arg-II promotes melanoma metastasis-related processes, such as melanoma cell migration and adhesion on endothelial cells, independently of its enzymatic activity. Moreover, the treatment of the cells with STAT3 inhibitor suppressed Arg-II-promoted melanoma cell migration and adhesion. Furthermore, catalase, but not superoxide dismutase, prevented STAT3 activation as well as increased melanoma cell migration and adhesion induced by overexpressing native or the inactive Arg-II. Taken together, our study uncovers both activity-dependent and independent mechanisms of Arg-II in promoting melanoma progression. While Arg-II enhances melanoma cell proliferation through polyamine dependently of its enzymatic activity, it promotes metastasis-related processes, that is, migration and adhesion onto endothelial cell, through mitochondrial H2 O2 -STAT3 pathway independently of the enzymatic activity. Suppressing Arg-II expression rather than inhibiting its enzymatic activity may, therefore, represent a novel strategy for the treatment of melanoma. |
Databáze: | OpenAIRE |
Externí odkaz: |