Long-lasting allergic patch test reactions to nickel sulfate: analysis by nickel quantification and immunocytochemistry
| Autor: | J. Handley, D. J. Todd, O.M. Dolan, Dorothy McMaster, G.E. Allen, D. Burrows, M. Walsh |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Time Factors CD4 antigen Microgram Biopsy Dermatology chemistry.chemical_compound Immune system Dermis Antigen Nickel medicine Immunology and Allergy Humans Hypersensitivity Delayed Fluorescent Antibody Technique Indirect Skin Immunity Cellular Epidermis (botany) Dose-Response Relationship Drug Chemistry Papillary dermis Spectrophotometry Atomic Patch Tests Molecular biology Immunohistochemistry medicine.anatomical_structure Immunology Female CD8 |
| Zdroj: | Contact dermatitis. 34(2) |
| ISSN: | 0105-1873 |
| Popis: | We previously showed the median duration of positive patch test reactions to nickel sulfate (5% pet.) was 9 days, and defined as long-lasting (LLAPTR) the 14.3% of reactions that persisted for 17 days or longer. The pathomechanisms of LLAPTR are unclear, but may involve either localized antigen persistence or abnormal downregulation of the cellular immune response. In this study, we compared (a) the nickel concentration and (b) the immunocytochemical nature of the local immune reaction, between biopsies from LLAPTR (n = 8) and normally resolving allergic patch test reactions (NRAPTR) (n = 8) to nickel sulfate. The concentration of nickel in LLAPTR (median 0.56 microgram/g, range 0.25-3.87 micrograms/g, mean 0.83 microgram/g, 95% CI 0.35-1.31) and NRAPTR (median 0.58 microgram/g, range 0.2-1.85 micrograms/g, mean 0.88 microgram/g, 95% CI 0.02-1.74) was similar. Activated T lymphocytes, expressing surface IL-2 receptor, HLA DR, DR alpha 1, DP, DQ, and CD2 > CD8 > CD4 antigens, were seen throughout the dermis and occasionally infiltrating the suprabasal layer of the epidermis in all biopsies. CD1 and HLA DR, DR alpha 1, DP, and DQ-expressing Langerhans cells were present throughout the epidermis and occasionally seen in the papillary dermis. HLA DR, DR alpha 1, DP, and DQ antigen expression were also seen on the surface of non-dendritic cells in the epidermis (probably either keratinocytes or T lymphocytes) and vascular endothelial cells in the papillary dermis. There were no significant qualitative or quantitative differences in the immunocytochemical nature of the localized immune reaction between LLAPTR and NRAPTR. These findings suggest that the pathomechanism of LLAPTR to nickel sulfate is unlikely to be explained simply on the basis of nickel concentration or the nature of the localized immune reaction at the patch test site. |
| Databáze: | OpenAIRE |
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