Mycotoxin Biosensor Based on Optical Planar Waveguide
| Autor: | Aleksey Nabok, Eszter Takács, Ali Madlool Al-Jawdah, Alan Holloway, Anna Tsargorodska, András Székács, Radhyah Mahdi Shaker Jarrah |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
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Materials science Optical Phenomena Health Toxicology and Mutagenesis planar waveguide lcsh:Medicine Physics::Optics 02 engineering and technology Biosensing Techniques Toxicology 01 natural sciences Article chemistry.chemical_compound Planar mycotoxins Mycotoxin Staphylococcal Protein A Immunoassay optical biosensor business.industry 010401 analytical chemistry lcsh:R polarization interferometer Antibodies Monoclonal Optical biosensor refractive index sensitivity 021001 nanoscience & nanotechnology Polarization (waves) Ochratoxins 0104 chemical sciences Interferometry Immobilized Proteins chemistry Optoelectronics 0210 nano-technology business Biosensor Refractive index |
| Zdroj: | Toxins Volume 10 Issue 7 Toxins, Vol 10, Iss 7, p 272 (2018) |
| ISSN: | 2072-6651 |
| Popis: | The research aim of this work is to develop a simple and highly sensitive optical biosensor for detection of mycotoxins. This sensor is built on a planar waveguide operating on the polarization interferometry principle, i.e., detecting a phase shift between p- and s-components of polarized light developed during the binding of analyte molecules. The operation of the proposed sensor is similar to that of a Mach&ndash Zehnder interferometer, while its design is much simpler and it does not require splitting the waveguide into two arms. The refractive index sensitivity of the polarization interferometer sensor was in the range of 5200 radians per refractive index unit (RIU). Several tests were conducted to detect ochratoxin A (OTA) at different concentrations in direct immunoassay with specific antibodies immobilized in the sensing window. The lowest concentration of OTA of 0.01 ng/mL caused a phase shift of nearly one period. The results obtained prove high sensitivity of the sensors, which are capable of detecting even lower concentrations of mycotoxins at the ppt (part-per-trillion) level. |
| Databáze: | OpenAIRE |
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