From Circulation to Cultivation: Plasmodium In Vivo versus In Vitro
| Autor: | Jennifer L. Guler, Audrey C. Brown |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Plasmodium Erythrocytes 030231 tropical medicine Virulence Malaria morbidity In Vitro Techniques Biology Host-Parasite Interactions 03 medical and health sciences 0302 clinical medicine In vivo parasitic diseases Animals Humans Cells Cultured biology.organism_classification Phenotype In vitro Malaria 030104 developmental biology Infectious Diseases Immunology Parasitology Adaptation Ex vivo |
| Zdroj: | Trends in Parasitology. 36:914-926 |
| ISSN: | 1471-4922 |
| DOI: | 10.1016/j.pt.2020.08.008 |
| Popis: | Research on Plasmodium parasites has driven breakthroughs in reducing malaria morbidity and mortality. Experimental analysis of in vivo/ex vivo versus in vitro samples serve unique roles in Plasmodium research. However, these distinctly different environments lead to discordant biology between parasites in host circulation and those under laboratory cultivation. Here, we review how in vitro factors, such as nutrient levels and physical forces, differ from those in the human host and the resulting implications for parasite growth, survival, and virulence. Additionally, we discuss the current utility of direct-from-host methodologies, which avoid the potentially confounding effects of in vitro cultivation. Finally, we make the case for methodological improvements that will drive research progress of physiologically relevant phenotypes. |
| Databáze: | OpenAIRE |
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