Vitamin D nanoemulsion enhances hepatoprotective effect of conventional vitamin D in rats fed with a high-fat diet
| Autor: | Mohamed El-Sherbiny, Mohamed El-Dosoky, Hany A. Elkattawy, Gamal Othman, Nehal M. Elsherbiny, Mohamed El-Shafey, Tamer Bedir |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vitamin Male medicine.medical_specialty NF-E2-Related Factor 2 Enzyme-Linked Immunosorbent Assay Hyperlipidemias Toxicology Diet High-Fat Protective Agents 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission Internal medicine medicine Vitamin D and neurology Animals Aspartate Aminotransferases Vitamin D Liver injury medicine.diagnostic_test Carnitine O-Palmitoyltransferase Chemistry Caspase 3 Tumor Necrosis Factor-alpha Fatty liver Lipid metabolism Alanine Transaminase General Medicine medicine.disease Interleukin-10 Nanostructures Rats 030104 developmental biology Fat-Soluble Vitamin Endocrinology Liver Receptors Calcitriol 030211 gastroenterology & hepatology Emulsions Liver function Lipid profile |
| Zdroj: | Chemico-biological interactions. 288 |
| ISSN: | 1872-7786 |
| Popis: | Background Non-alcoholic fatty liver disease (NAFLD) is associated with hyperlipidemia, obesity and type II diabetes. Due to increasing prevalence of these diseases globally, NAFLD is considered as a common form of chronic liver diseases. Vitamin D is a fat soluble vitamin with reported anti-inflammatory, anti-oxidant and immune modulating activity. Hypovitaminosis D often coexists with NAFLD and various studies reported beneficial role of vitamin D in modulating NAFLD. However, variable oral bioavailability, poor water solubility, and chemical degradation hinder the clinical application of vitamin D. Purpose We evaluated the potential protective effect of Vitamin D nanoemulsion (developed by sonication and pH-Shifting of pea protein isolate and canola oil) compared to conventional vitamin D against liver injury in rats fed with high fat diet (HFD). Methods We analyzed liver function enzymes, lipid profile, lipid metabolism, levels and histopathology of inflammation and fibrosis in rat liver tissues. Results HFD fed rats exhibited deterioration of liver function, poor lipid profile, decreased fatty acid oxidation and up-regulation of inflammatory cytokines and extracellular matrix deposition. Vitamin D administration reduced elevated liver enzymes, improved lipid profile, enhanced fatty acid oxidation and attenuated liver inflammation and fibrosis. Interestingly, vitamin D nanoemulsion was superior to conventional vitamin D with remarkable hepatoprotective effect against HFD-induced liver injury. Conclusion This study demonstrated vitamin D nanoemulsion as a more efficient formulation with more prominent hepatoprotective effect against HFD-induced liver injury compared to conventional oral vitamin D. |
| Databáze: | OpenAIRE |
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