Altered Expression of TFF-1 and CES-2 in Barrett's Esophagus and Associated Adenocarcinomas

Autor: Lisa M. Sapinoso, Wanghai Zhang, Christopher A. Moskaluk, Gina R. Petroni, Steven M. Powell, Garret M. Hampton, Charles A. Fox, Howard L. McLeod, Hong Zhang, Henry F. Frierson, Tarun Mullick
Rok vydání: 2005
Předmět:
Cancer Research
Pathology
Oligonucleotides
Carboxylesterase 2
esophageal or gastric adenocarcinoma
RNA
Complementary
Mice
Oligonucleotide Array Sequence Analysis
Aged
80 and over
Stomach
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Immunohistochemistry
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Multigene Family
Adenocarcinoma
Trefoil Factor-1
Signal Transduction
Research Article
Adult
medicine.medical_specialty
Trefoil Factor 1
Mice
Nude
CD13 Antigens
Biology
Malignancy
lcsh:RC254-282
Barrett Esophagus
Barrett's esophagus
Esophagus
tissue microarry
Biomarkers
Tumor
medicine
Animals
Humans
Gene
Aged
Models
Genetic
Gene Expression Profiling
Tumor Suppressor Proteins
medicine.disease
Gene expression profiling
Gastric Mucosa
RNA
Carboxylic Ester Hydrolases
Precancerous Conditions
Neoplasm Transplantation
Zdroj: Neoplasia: An International Journal for Oncology Research, Vol 7, Iss 4, Pp 407-416 (2005)
ISSN: 1476-5586
DOI: 10.1593/neo.04715
Popis: Identification of biomarkers to recognize individuals with Barrett's esophagus (BE) predisposed to develop malignancy is currently a pressing issue. We utilized gene expression profiling to compare molecular signatures of normal esophagus and stomach, BE, and adenocarcinoma (AC) to identify such potential biomarkers. Over 22,000 genes were analyzed by oligonucleotide microarrays on 38 unique RNA Unsupervised and supervised clusterings were performed on a subset of 2849 genes that varied most significantly across the specimens. Immunohistochemistry (IHC) for two of the significantly differentially expressed gene products was performed on tissue microarrays. Unsupervised clustering identified two discernable molecular BE profiles, one of which was similar to normal gastric tissue ("BE1"), and another that was shared by several of the AC specimens ("BE2"). The BE1 profile included expression of several genes that have been described as tumor-suppressor genes, most notably trefoil factor 1 (TFF-1). The BE2 profile included expression of genes previously found overexpressed in cancers, such as carboxylesterase-2 (CES-2). IHC demonstrated the loss of TFF-1 late in the progression of BE to AC. It also revealed CES-2 as being upregulated in AC documented to have arisen in the presence of BE. These potential biomarkers, as well as the relative expression of genes from BE1 versus those from BE2, may be validated in the future to aid in risk stratification and guide treatment protocols in patients with BE and associated AC.
Databáze: OpenAIRE
Externí odkaz: