Early monitoring of acute tubular necrosis in the rat kidney by 23Na-MRI
| Autor: | Andriy M. Babsky, Bruce A. Molitoris, Carrie L. Phillips, Bharath K. Atthe, Navin Bansal, Paige Hopewell |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Kidney Cortex Magnetic Resonance Spectroscopy Physiology Urinary system Sodium Ischemia chemistry.chemical_element Kidney medicine Image Processing Computer-Assisted Animals Rats Wistar Medulla Acute tubular necrosis Kidney Medulla Renal sodium reabsorption Reabsorption Anatomy Articles Kidney Tubular Necrosis Acute medicine.disease Magnetic Resonance Imaging Rats medicine.anatomical_structure chemistry Data Interpretation Statistical Sodium Isotopes Algorithms |
| Popis: | Reabsorption of water and other molecules is dependent on the corticomedullary sodium concentration gradient in the kidney. During the early course of acute tubular necrosis (ATN), this gradient is altered. Therefore,23Na magnetic resonance imaging (MRI) was used to study the alterations in renal sodium distribution in the rat kidney during ischemia and reperfusion (IR) injury, which induces ATN. In-magnet ischemia was induced for 0 (control), 10, 20, 30 or 50 min in Wistar rats.23Na images were collected every 10 min during baseline, ischemia, and 60-min reperfusion periods. T1and T2relaxation times were measured by both23Na-MRI and -MRS on a separate cohort of animals during ischemia and reperfusion for correction of relaxation-related tissue sodium concentration (TSC). A marked decrease was observed in the medulla and cortex23Na-MRI signal intensity (SI) during the early evolution of ATN caused by IR injury, with the sodium reabsorption function of the kidney being irreversibly damaged after 50 min of ischemia. Sodium relaxation time characteristics were similar in the medulla and cortex of normal kidney, but significantly decreased with IR. The changes in relaxation times in both compartments were identical; thus the medulla-to-cortex sodium SI ratio represents the TSC ratio of both compartments. The extent of IR damage observed with histological examination correlated with the23Na-MRI data.23Na-MRI has great potential for noninvasive, clinical diagnosis of evolving ATN in the setup of acute renal failure and in differentiating ATN from other causes of renal failure where tubular function is maintained. |
| Databáze: | OpenAIRE |
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