Expression of tight and adherens junction proteins in cervical neoplasia
| Autor: | Ciaran Cunniffe, Brenda Brankin, Helen Lambkin, Fergus Ryan |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Immunology Uterine Cervical Neoplasms Cervical intraepithelial neoplasia Occludin Microbiology Tight Junctions Adherens junction medicine Humans Immunology and Allergy Claudin Epithelial polarity Tight Junction Proteins Tight junction Chemistry Biochemistry (medical) Adherens Junctions medicine.disease Molecular biology Epithelium Infectious Diseases medicine.anatomical_structure Cell culture Carcinoma Squamous Cell Female HeLa Cells |
| Zdroj: | British Journal of Biomedical Science. 69:147-153 |
| ISSN: | 0967-4845 |
| DOI: | 10.1080/09674845.2012.12069143 |
| Popis: | Adherens junctions (AD and tight junctions (TJ) play a key role in maintaining the apical-basolateral polarity and cohesive structure of epithelial cells. These epithelial junctions are maintained by the interaction of several key proteins including E-cadherin, claudins, occludin and zona-occludens. The zinc finger protein, Snail, has previously been identified as a possible regulator of several of these AJ and TJ proteins. Expression levels of ZO-1, occludin, claudins, E-cadherin, human Scribble protein and Snail were determined in HeLa and CaSki cervical carcinoma cell lines by imuunohistochemistry (IHC) and Western blotting. In tandem, tissue microarrays were utilised in the IHC-based detection of E-cadherin in 26 cases of non-cancerous cervical tissue, 15 cases of cervical intraepithelial neoplasia 1 (CIN1), 11 cases of CIN2, 12 cases of CIN3, 50 cases of squamous cell carcinoma and two cases of adenocarcinoma. This study found aberrant E-cadherin expression in CIN lesions and cases of squamous cell carcinoma compared to normal epithelium. HeLa cells were E-cadherin-negative while CaSki cells were positive, with HeLa cells showing a high level of Snail expression. Occludin and ZO-1 expression was detected in both cell lines. No expression was observed for claudin 1 or claudin 5 tight junction proteins in HeLa cells or CaSki cells. Loss of expression of claudin 1, claudin 5 and E-cadherin, with concomitant increase in Snail may be associated with loss of epithelial cell polarity and alterations in intercellular adhesion. |
| Databáze: | OpenAIRE |
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