Oscillations and variability in the p53 system
| Autor: | Talia Yarnitzky, Yuvalal Liron, Nitzan Rosenfeld, Shalev Itzkovitz, Paz Polak, Ron Milo, Uri Alon, Naama Geva-Zatorsky, Alex Sigal, Erez Dekel, Galit Lahav |
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| Rok vydání: | 2005 |
| Předmět: |
Cell division
Period (gene) Cell cancer genetics Biology fluorescence microscopy General Biochemistry Genetics and Molecular Biology Article Biological Clocks Negative feedback Cell Line Tumor medicine Humans News and Views Feedback Physiological General Immunology and Microbiology Applied Mathematics Dynamics (mechanics) quantitative biology systems biology Dose-Response Relationship Radiation Proto-Oncogene Proteins c-mdm2 Models Theoretical Cell biology medicine.anatomical_structure Amplitude Computational Theory and Mathematics Cell culture Gamma Rays Female Tumor Suppressor Protein p53 General Agricultural and Biological Sciences Noise (radio) Information Systems DNA Damage |
| Zdroj: | Molecular Systems Biology |
| ISSN: | 1744-4292 |
| Popis: | Understanding the dynamics and variability of protein circuitry requires accurate measurements in living cells as well as theoretical models. To address this, we employed one of the best-studied protein circuits in human cells, the negative feedback loop between the tumor suppressor p53 and the oncogene Mdm2. We measured the dynamics of fluorescently tagged p53 and Mdm2 over several days in individual living cells. We found that isogenic cells in the same environment behaved in highly variable ways following DNA-damaging gamma irradiation: some cells showed undamped oscillations for at least 3 days (more than 10 peaks). The amplitude of the oscillations was much more variable than the period. Sister cells continued to oscillate in a correlated way after cell division, but lost correlation after about 11 h on average. Other cells showed low-frequency fluctuations that did not resemble oscillations. We also analyzed different families of mathematical models of the system, including a novel checkpoint mechanism. The models point to the possible source of the variability in the oscillations: low-frequency noise in protein production rates, rather than noise in other parameters such as degradation rates. This study provides a view of the extensive variability of the behavior of a protein circuit in living human cells, both from cell to cell and in the same cell over time. |
| Databáze: | OpenAIRE |
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