Prevention of mother-to-child HIV-1 transmission in Burkina Faso: evaluation of vertical transmission by PCR, molecular characterization of subtypes and determination of antiretroviral drugs resistance
| Autor: | Jacques Simpore, Joseph D. Sia, Jean-Baptiste Nikiema, Adjirita Koama, Tegewende R. Compaore, Thérèse Kagoné, Tani Sagna, Zoenabo Douamba, Virginio Pietra, Valérie Jean Telesphore Bazie, M. Zeba, Charlemagne Gnoula, Djeneba Ouermi, Cyrille Bisseye, Remy Moret, Florencia Djigma, Catherine M Pirkle |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
medicine.medical_specialty HAART pregnant women sequencing genotypes mutations Adolescent Population Mothers HIV Infections Drug resistance Polymerase Chain Reaction Virus Young Adult Pregnancy Internal medicine Antiretroviral Therapy Highly Active Genotype Burkina Faso Drug Resistance Viral medicine Prevalence Humans Pregnancy Complications Infectious education education.field_of_study Transmission (medicine) business.industry Health Policy Public health lcsh:Public aspects of medicine Public Health Environmental and Occupational Health Infant virus diseases lcsh:RA1-1270 Viral Load Resistance mutation Virology Infectious Disease Transmission Vertical CD4 Lymphocyte Count Residual risk Mutation HIV-1 Original Article Female business |
| Zdroj: | Global Health Action; Vol 8 (2015): incl Supplements Global Health Action Global Health Action, Vol 8, Iss 0, Pp 1-8 (2015) |
| ISSN: | 1654-9716 1654-9880 |
| Popis: | Background : Vertical human immunodeficiency virus (HIV) transmission is a public health problem in Burkina Faso. The main objective of this study on the prevention of mother-to-child HIV-1 transmission was to determine the residual risk of HIV transmission in infants born to mothers receiving highly active antiretroviral therapy (HAART). Moreover, we detect HIV antiretroviral (ARV) drug resistance among mother–infant pairs and identify subtypes and circulating recombinant forms (CRF) in Burkina Faso. Design : In this study, 3,215 samples of pregnant women were analyzed for HIV using rapid tests. Vertical transmission was estimated by polymerase chain reaction in 6-month-old infants born to women who tested HIV positive. HIV-1 resistance to ARV, subtypes, and CRFs was determined through ViroSeq kit using the ABI PRISM 3,130 sequencer. Results : In this study, 12.26% (394/3,215) of the pregnant women were diagnosed HIV positive. There was 0.52% (2/388) overall vertical transmission of HIV, with rates of 1.75% (2/114) among mothers under prophylaxis and 0.00% (0/274) for those under HAART. Genetic mutations were also isolated that induce resistance to ARV such as M184V, Y115F, K103N, Y181C, V179E, and G190A. There were subtypes and CRF of HIV-1 present, the most common being: CRF06_CPX (58.8%), CRF02_AG (35.3%), and subtype G (5.9%). Conclusions : ARV drugs reduce the residual rate of HIV vertical transmission. However, the virus has developed resistance to ARV, which could limit future therapeutic options when treatment is needed. Resistance to ARV therefore requires a permanent interaction between researchers, physicians, and pharmacists, to strengthen the network of monitoring and surveillance of drug resistance in Burkina Faso. Keywords : pregnant women; HAART; sequencing; genotypes; mutations (Published: 27 January 2015) Citation : Glob Health Action 2015, 8 : 26065 - http://dx.doi.org/10.3402/gha.v8.26065 |
| Databáze: | OpenAIRE |
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