Histochemically detected enzymatic alterations in rat lung exposed to ozone
| Autor: | W.S. Tyler, William L. Castleman, D. L. Dungworth |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
Pathology medicine.medical_specialty ATPase Clinical Biochemistry Peripheral blood mononuclear cell Pathology and Forensic Medicine Electron Transport Complex IV Ozone Diaphorase medicine Cytochrome c oxidase Animals Molecular Biology Lung Dihydrolipoamide Dehydrogenase Adenosine Triphosphatases Dihydrolipoamide dehydrogenase biology L-Lactate Dehydrogenase Staining and Labeling Chemistry Histocytochemistry Esterases respiratory system Alkaline Phosphatase NAD Epithelium Rats medicine.anatomical_structure biology.protein Alkaline phosphatase Oxidation-Reduction NADP |
| Zdroj: | Experimental and molecular pathology. 19(3) |
| ISSN: | 0014-4800 |
| Popis: | Enzymatic activities were studied histochemically in lungs from young rats which had been exposed to 0.8 ppm ozone continuously for 7 days. Walls of terminal airways and interalveolar septa in centriacinar regions were the areas of the lung most noticeably affected by ozone exposure. Histologically these structures were observed to be thickened and infiltrated by mononuclear cells. Bronchiolar epithelium in infiltrated areas had lower NADH- and NADPH-diaphorase reactivities and higher ATPase reactivity than similar epithelium in control lungs. Interalveolar septa in centriacinar regions of ozone-exposed lungs had increased diaphorase, LDH, ATPase, and cytochrome oxidase reactivities. Increased septal histochemical reactivity was observed not only in the centriacinar zone of septa that were thickened and infiltrated, but also in an immediately adjacent zone of septa that were histologically unaffected. The increases in enzymatic reactivity could be part of a protective mechanism operating locally in centriacinar regions of ozone-exposed lungs to help maintain cellular integrity. |
| Databáze: | OpenAIRE |
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