Density gradient ultracentrifugation of rubella virus
| Autor: | Parkman Pd, Thomas E. Hobbins, Marvin S. Amstey |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Infectivity
Sucrose Strain (chemistry) Density gradient Virulence Sulfates viruses Buoyant density Cesium Hemagglutinins Viral Rubella virus Biology medicine.disease_cause Virology General Biochemistry Genetics and Molecular Biology Virus Surface-Active Agents Chlorides Cell culture medicine Centrifugation Density Gradient Density gradient ultracentrifugation Antigens Ethers |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 127(4) |
| ISSN: | 0037-9727 |
| Popis: | SummaryThe buoyant density of rubella virus HA was determined to be 1.23 in Cs2SO4. A density of 1.33 was obtained in CsCl. Rubella virus HA, either Tween-80. ether-treated or untreated, and infectivity could not be separated on sucrose by rate zonal or density gradient techniques. These activities also could not be separated on CsCl and Cs2SO4 density gradients. The banding density of rubella virus HA preparations was unaffected by the type of cell culture used for antigen production; neither the passage level nor the strain of virus altered the point at which rubella virus HA banded in CsCl. |
| Databáze: | OpenAIRE |
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