Allelic heterogeneity of HLA-B35 subtypes in different populations as assessed by DNA typing
| Autor: | Marcelo Fernandez-Vina, N. Lindel, M. L. Satz, C. Gorodezky, G. del C. Theiler, Peter Stastny, Yanina C. Marcos, Leonardo Fainboim, M. Capucchio |
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| Rok vydání: | 1995 |
| Předmět: |
Immunology
Molecular Sequence Data Genes MHC Class I Human leukocyte antigen Biology Biochemistry Polymerase Chain Reaction law.invention law Genetics Immunology and Allergy Humans Typing Allele Polymerase chain reaction Alleles DNA Primers Base Sequence Histocompatibility Testing Haplotype Nucleic Acid Hybridization General Medicine Histocompatibility Transplantation Allelic heterogeneity HLA-B35 Antigen |
| Zdroj: | Tissue antigens. 46(3 ( Pt 1)) |
| ISSN: | 0001-2815 |
| Popis: | HLA-B35, a class I antigen differentially associated to several diseases in different ethnic groups, comprises at least eight alleles which differ among them by one to six amino acids. In the present work a rapid DNA typing procedure was used to investigate the distribution of the various HLA-B35 alleles in different populations. The approach is based on a group-specific PCR amplification of a set of closely related HLA-B alleles sharing a Thr in position 45 of the alpha-1 domain. The amplified DNA was then hybridized to a panel of sequence-specific oligonucleotide (SSO) probes designed to recognize the polymorphic residues in previously reported HLA-B35 subtypes. This methodology was successfully tested in 100 individuals of four different populations, previously typed by serology as HLA-B35, and in six reference panel cells of the 10th International Histocompatibility Workshop. HLA-B*3501 was the predominant subtype in all populations. B*3502, B*3503 and, to a lesser extent B*3508, were also found. Among Mexican Mestizos, thirteen individuals had patterns of SSO hybridization suggestive of new B35 alleles. The evolutionary considerations on the different B35 alleles and their extended B35,Cw4 haplotypes are discussed. |
| Databáze: | OpenAIRE |
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