Caspases and thrombin activity regulation by specific serpin inhibitors in bovine skeletal muscle
| Autor: | Abdelghani Boudjellal, Yasmine Boudida, Samira Becila, Miguel Angel Sentandreu, Kahina Hafid, Mohammed Gagaoua, Ahmed Ouali, Brigitte Picard |
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| Přispěvatelé: | Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université des Frères Mentouri (Constantine 1), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Proteases
myoblast differentiation Serine Proteinase Inhibitors [SDV.BIO]Life Sciences [q-bio]/Biotechnology Molecular Sequence Data Bioengineering Serpin Biology Applied Microbiology and Biotechnology Biochemistry Thrombin medicine serine proteases inhibitors Animals Myocyte Amino Acid Sequence Muscle Skeletal Molecular Biology Myogenesis Antithrombin serpins apoptosis Skeletal muscle General Medicine bovine skeletal muscle Kinetics antithrombin medicine.anatomical_structure Apoptosis Caspases Cattle Biotechnology medicine.drug |
| Zdroj: | Applied Biochemistry and Biotechnology Applied Biochemistry and Biotechnology, Humana Press, 2015, 177, pp.279-303. ⟨10.1007/s12010-015-1762-4⟩ |
| ISSN: | 0273-2289 |
| DOI: | 10.1007/s12010-015-1762-4⟩ |
| Popis: | International audience; In living cells, after activation, protein inhibitors constitute the last step of proteases activity regulation. This review intends to provide original information about a group of bovine muscle serine proteases inhibitors belonging to the Serpin superfamily and characterized at the gene and protein level. This report is the only one and the first to provide much information on this group of proteases inhibitors of the serpin type and their potential biological functions. Amongst the eight genes identified in bovine, three serpins were purified from the muscle tissue and characterized. These are two members of the bovSERPINA3 family, i.e., bovSERPINA3-1 and A3-3, and the last one is antithrombin III (AT-III or BovSERPINC1). BovSERPINA3 family comprises at least eight protein members encoded by different genes mapped on chromosome 7q23–q26 cluster. BovSERPINA3-1 and A3-3 were shown to locate within muscle cells and are cross-class inhibitors strongly active against trypsin as well as against human initiator and effector caspases 8 and 3. They constitute a key apoptosis control in mammals. They were thus expressed in proliferating and confluent myoblasts phases where cells must be alive but not in myotubes. Antithrombin III inhibits trypsin and, in a heparin dependent manner, thrombin. AT-III and its mRNA were expressed in muscle cells and in differentiating primary myoblasts in culture. |
| Databáze: | OpenAIRE |
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