Aliesterase Activity in Normal and Postheparin Human Blood Sera
| Autor: | Jiri Skorepa, Oyin Somorin |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Physostigmine
Sodium chemistry.chemical_element Biochemistry Esterase Substrate Specificity Fluorides chemistry.chemical_compound Organophosphorus Compounds Column chromatography Sodium fluoride medicine Cholinesterases Humans Molecular Biology chemistry.chemical_classification Chromatography Human blood Heparin Chemistry Osmolar Concentration General Medicine Hydrogen-Ion Concentration Assay technique Butyrates Enzyme Laurates medicine.drug |
| Zdroj: | The Journal of Biochemistry. 83:617-623 |
| ISSN: | 1756-2651 0021-924X |
| Popis: | An enzyme with characteristics typical of aliesterase has been found in human blood serum using a gas solid chromatographic assay technique. This conflicts with the findings of several authors that aliesterase is absent in the human blood. Another aliesterase is released into the blood stream after intravenous administration of heparin. Partial purification of the aliesterase in normal (preheparin) and postheparin sera was effected by column chromatography using CM- and DEAE-Sephadex. The preheparin aliesterase and postheparin aliesterase have different pH optima of 7.0 and 8.5 respectively. The preheparin aliesterase activity was very sensitive to sodium fluoride and insensitive to a negatively charged detergent, sodium lauryl sulfate, unlike the postheparin esterase which was highly sensitive to sodium lauryl sulfate and comparatively less sensitive to sodium fluoride. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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