An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica
| Autor: | Laurence Vernis, J A Huberman, M. Chasles, A Abbas, Christine Brun, Claude Gaillardin, Philippe Fournier |
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| Přispěvatelé: | Laboratoire de génétique moléculaire et cellulaire, Institut National de la Recherche Agronomique (INRA), Roswell Park Cancer Institute [Buffalo], Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Spinelli, Lionel, Roswell Park Cancer Institute [Buffalo] (RPCI) |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
DNA Replication
[SDV]Life Sciences [q-bio] Centromere Genetic Vectors Molecular Sequence Data Replication Origin Biology [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Origin of replication 03 medical and health sciences Plasmid Consensus Sequence Cloning Molecular DNA Fungal Molecular Biology Chromosomal Deletion ComputingMilieux_MISCELLANEOUS [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM] 030304 developmental biology Genetics 0303 health sciences Base Sequence 030302 biochemistry & molecular biology DNA replication Fungal genetics Chromosome Chromosome Mapping Yarrowia [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology biology.organism_classification Molecular biology 13. Climate action Saccharomycetales Chromosomes Fungal [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] Plasmids Research Article |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 1997, 17 (4), pp.1995-2004 Molecular and Cellular Biology, 1997, 17 (4), pp.1995-2004 |
| ISSN: | 0270-7306 1098-5549 |
| Popis: | Two DNA fragments displaying ARS activity on plasmids in the yeast Yarrowia lipolytica have previously been cloned and shown to harbor centromeric sequences (P. Fournier, A. Abbas, M. Chasles, B. Kudla, D. M. Ogrydziak, D. Yaver, J.-W. Xuan, A. Peito, A.-M. Ribet, C. Feynerol, F. He, and C. Gaillardin, Proc. Natl. Acad. Sci. USA 90:4912-4916, 1993; and P. Fournier, L. Guyaneux, M. Chasles, and C. Gaillardin, Yeast 7:25-36, 1991). We have used the integration properties of centromeric sequences to show that all Y. lipolytica ARS elements so far isolated are composed of both a replication origin and a centromere. The sequence and the distance between the origin and centromere do not seem to play a critical role, and many origins can function in association with one given centromere. A centromeric plasmid can therefore be used to clone putative chromosomal origins coming from several genomic locations, which confer the replicative property on the plasmid. The DNA sequences responsible for initiation in plasmids are short (several hundred base pairs) stretches which map close to or at replication initiation sites in the chromosome. Their chromosomal deletion abolishes initiation, but changing their chromosomal environment does not. |
| Databáze: | OpenAIRE |
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