Metabolite Profiling and Transcriptome Analysis Provide Insight into Seed Coat Color in Brassica juncea

Autor: Yunshan Tang, Guoxia Shang, Nengwen Yin, Ran Hu, Shulin Shen, Xueqin Liu, Si Chen, Chao Zhang, Yuanyi Mao, Cunmin Qu, Kun Lu, Jiana Li, Liezhao Liu, Fujun Sun
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Brassica
01 natural sciences
Catechin
Transcriptome
Gene Expression Regulation
Plant
Brassica juncea L
Biology (General)
Spectroscopy
TRANSPARENT TESTA 8
chemistry.chemical_classification
biology
Pigmentation
Gene Expression Regulation
Developmental
food and beverages
General Medicine
Hydroxycinnamic acid
Computer Science Applications
Chemistry
Phenotype
expression patterns
Seeds
Metabolome
Mustard Plant
Coat
QH301-705.5
Glucosinolates
Vegetable crops
Genes
Plant
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Botany
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Gene
Gene Expression Profiling
Organic Chemistry
metabolic profiling
biology.organism_classification
030104 developmental biology
chemistry
Metabolite profiling
flavonoids
transcriptome
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 13
International Journal of Molecular Sciences, Vol 22, Iss 7215, p 7215 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22137215
Popis: The allotetraploid species Brassica juncea (mustard) is grown worldwide as oilseed and vegetable crops
the yellow seed-color trait is particularly important for oilseed crops. Here, to examine the factors affecting seed coat color, we performed a metabolic and transcriptomic analysis of yellow- and dark-seeded B. juncea seeds. In this study, we identified 236 compounds, including 31 phenolic acids, 47 flavonoids, 17 glucosinolates, 38 lipids, 69 other hydroxycinnamic acid compounds, and 34 novel unknown compounds. Of these, 36 compounds (especially epicatechin and its derivatives) accumulated significantly different levels during the development of yellow- and dark-seeded B. juncea. In addition, the transcript levels of BjuDFR, BjuANS,BjuBAN, BjuTT8, and BjuTT19 were closely associated with changes to epicatechin and its derivatives during seed development, implicating this pathway in the seed coat color determinant in B. juncea. Furthermore, we found numerous variations of sequences in the TT8A genes that may be associated with the stability of seed coat color in B. rapa, B. napus, and B. juncea, which might have undergone functional differentiation during polyploidization in the Brassica species. The results provide valuable information for understanding the accumulation of metabolites in the seed coat color of B. juncea and lay a foundation for exploring the underlying mechanism.
Databáze: OpenAIRE
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