Successful establishment and evaluation of a new animal model for studying the hepatitis B virus YVDD mutant
| Autor: | Xu Teng, Sijia Chen, Yan-Xiu Ma, Wei-Zhen Xu, Zhi-Wei Song, Li-Juan Fu, Hong-Xi Gu, Mei-Li Hao |
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| Rok vydání: | 2012 |
| Předmět: |
Gene Expression Regulation
Viral HBsAg Hepatitis B virus Mutant Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Mice Plasmid In vivo Virology medicine Animals Hepatitis B e Antigens RNA Messenger Mice Inbred BALB C Hepatitis B Surface Antigens virus diseases RNA-Directed DNA Polymerase General Medicine Hepatitis B Molecular biology digestive system diseases Specific Pathogen-Free Organisms HBcAg Disease Models Animal Viral replication HBeAg Liver Hepatitis Viral Animal Mutation Female |
| Zdroj: | Archives of virology. 158(4) |
| ISSN: | 1432-8798 |
| Popis: | The treatment of infection with lamivudine-resistant mutants of hepatitis B virus (HBV) with mutations in the YMDD motif has become a crucial issue in the clinic. In this work, the plasmids pcDNA3.1 (+)-HBV/C-YVDD and pcDNA3.1 (+)-HBV/C-YMDD were constructed and injected into BALB/c mice using a hydrodynamics-based procedure to investigate viral replication and expression of HBV lamivudine-resistant YVDD mutants in vivo. Compared with the YMDD group, HBsAg levels were higher in sera of mice in the YVDD group, but HBeAg levels were lower on day 1 after injection. Levels of HBcAg in hepatocytes were higher in the YVDD group on day 1, whereas the HBsAg levels were lower. The levels of HBV mRNA in the liver were higher in mice in the YVDD group on day 1 after injection. The results showed that injection with these plasmids resulted in efficient initiation of replication of HBV in mice and also suggested that the combined mutations in YVDD mutants could affect the replication process. |
| Databáze: | OpenAIRE |
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