Design and Development of a Two-Color Emissive FRET Pair Based on a Photostable Fluorescent Deoxyuridine Donor Presenting a Mega-Stokes Shift
| Autor: | Benoît Y. Michel, Dominique Bonhomme, Nicolas Barthes, Delphine Debayle, Krishna Gavvala, Alain Burger, Yves Mély, Anne Sophie Dabert-Gay |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fluorophore
010405 organic chemistry Organic Chemistry Conjugated system 010402 general chemistry 01 natural sciences Combinatorial chemistry Fluorescence Acceptor Deoxyuridine 0104 chemical sciences chemistry.chemical_compound symbols.namesake Förster resonance energy transfer chemistry Stokes shift symbols Moiety |
| Zdroj: | The Journal of Organic Chemistry. 81:10733-10741 |
| ISSN: | 1520-6904 0022-3263 |
| DOI: | 10.1021/acs.joc.6b01807 |
| Popis: | We report the synthesis and site-specific incorporation in oligodeoxynucleotides (ODNs) of an emissive deoxyuridine analog electronically conjugated on its C5-position with a 3-methoxychromone moiety acting as a fluorophore. When incorporated in ODNs, this fluorescent deoxyuridine analog exhibits remarkable photostability and good quantum yields. This deoxyuridine analog also displays a mega-Stokes shift, which allows for its use as an efficient donor for FRET-based studies when paired with the yellow emissive indocarbocyanine Cy3 acceptor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|