Further studies on the glucose inhibition of β-1,3-glucanohydrolase increase during gut differentiation of sand dollar larvae
| Autor: | Laurence Jay Korn, Victor D. Vacquier, David Epel |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
medicine.medical_specialty
Time Factors Glycoside Hydrolases Cycloheximide chemistry.chemical_compound Transcription (biology) biology.animal Internal medicine medicine Animals Seawater Molecular Biology Sea urchin chemistry.chemical_classification Messenger RNA Larva Dose-Response Relationship Drug biology Cell Differentiation Embryo Cell Biology biology.organism_classification Intestines Glucose Endocrinology Enzyme chemistry Biochemistry Sand dollar Echinodermata Developmental Biology |
| Zdroj: | Developmental Biology. 36:1-7 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(74)90186-9 |
| Popis: | β-1,3-Glucanase activity in sand dollar larvae appears at 22 hr of development. The presence of glucose, the major product of this enzyme, in the ambient seawater depresses β-glucanase activity 3-fold. This glucose depression occurs only if glucose is present during the time of increase in β-glucanase activity. Maximum depression occurs at 10 mM glucose; the effect is not mimicked by l -glucose or methyl glucose analogs. The glucose effect is reversible up to 32 hr of development, but not thereafter. Studies on sea urchin larvae show that β-glucanase activity follows a similar pattern of increase in these embryos, but is not affected by exogenous glucose. The site of action of glucose may be at the synthesis of β-glucanase mRNA. If larvae are incubated in glucose for 6 hr and actinomycin D is substituted for glucose, no increased β-glucanase activity occurs. However, if larvae are incubated in cycloheximide and actinomycin D is substituted for cycloheximide, increased β-glucanase activity is observed. Thus glucose may be acting directly on transcription or it may be affecting the turnover of β-glucanase mRNA. |
| Databáze: | OpenAIRE |
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