Maintenance of head and neck tumor on-chip: gateway to personalized treatment?
| Autor: | Elena Kuvshinova, Laszlo Karsai, Nicholas D. Stafford, Stephen Crank, Ruth Bower, Dmitriy Kuvshinov, Victoria L. Green, John Greenman |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty tumor Cell microfluidic Biology Flow cytometry 03 medical and health sciences chemistry.chemical_compound Tissue culture 0302 clinical medicine medicine Propidium iodide tissue culture Head and neck medicine.diagnostic_test business.industry Head and neck cancer medicine.disease 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Trypan blue head and neck cancer Personalized medicine business Biotechnology Research Article |
| Zdroj: | Future Science OA |
| ISSN: | 2056-5623 |
| Popis: | Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/results: HNSCC (n = 15) was dissected (5–10 mg) and either analyzed immediately or cultured in a microfluidic device (37°C) for 48 h. No difference was observed in morphology between pre- and postculture specimens. Dissociated samples were analyzed using trypan blue exclusion (viability), propidium iodide flow cytometry (death) and MTS assay (proliferation) with no significant difference observed highlighting tissue maintenance. Computational fluid dynamics showed laminar flow within the system. Conclusion: The microfluidic culture system successfully maintained HNSCC for 48 h, the culture system will allow testing of different treatment modalities with response monitoring. Lay abstract Head and neck cancers often have a poor treatment outcome. In order to study the response of the tissue, a miniaturized culture system has been developed to keep a small piece of tumor alive. In the current study, we show that small pieces of cancer tissue can be maintained in the system, using tissue structure and viability of single cells as a guide. In future work, patient equivalent treatments can be applied to these microculture systems to investigate individual patient tumor responses, which could help to guide treatment selection. |
| Databáze: | OpenAIRE |
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