Microarray profiles of human basal cell carcinoma: insights into tumor growth and behavior
| Autor: | Binghe Wang, Irwin Freed, Nowell Solish, Chao Lu, Hideaki Watanabe, Brandon G. Howell, Daniel N. Sauder, Adam J. Mamelak |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Skin Neoplasms Microarray Caveolin 1 Kruppel-Like Transcription Factors Dermatology Biology Zinc Finger Protein Gli2 Biochemistry Caveolins Metastasis Extracellular matrix Gene expression medicine Phosphoprotein Phosphatases Humans Basal cell carcinoma Molecular Biology Gene Oligonucleotide Array Sequence Analysis integumentary system Gene Expression Profiling Nuclear Proteins Cell cycle medicine.disease Gene expression profiling DNA-Binding Proteins Carcinoma Basal Cell Cancer research Keratins Collagen |
| Zdroj: | Journal of dermatological science. 39(1) |
| ISSN: | 0923-1811 |
| Popis: | Summary Purpose: Basal cell carcinoma (BCC) is the most common human neoplasm. Much interest lies in determining the genetic basis of BCC to explain the unique locally invasive phenotype and infrequent metastatic behavior of these skin tumors. Objective: We sought to examine a gene expression profile for BCC to elucidate new molecules responsible for its unique growth characteristics. Methods: We analyzed gene expression patterns of 50 BCC tumors using spotted cDNA microarrays of 1718 characterized human genes related to cancer and immunity. This is the largest and most comprehensive gene expression study ever performed for BCC. Nodular and sclerosing histological subtypes of BCC were examined and compared to normal control skin. After statistical filtering, 374 significantly dysregulated genes were sorted by hierarchical clustering to determine trends of gene expression and similarities between patient gene expression profiles. Results: A total of 165 upregulated genes and 115 downregulated genes were identified. These covered a range of categories, including extracellular matrix, cell junctions, motility, metastasis, oncogenes, tumor suppressors, DNA repair, cell cycle, immune regulation and angiogenesis. Clusters of genes were either commonly dysregulated across the 50 patient sample, or selectively affected in subsets of tumors. Histological subtypes were not distinguishable by hierarchical clustering. Many of the genes elucidated, including collagen type IV subunits and other novel candidates, possess functions related to extracellular matrix remodeling and metastasis. Conclusion: These results suggest a gene profile which may explain the invasive growth yet rarely metastatic behavior of BCC. The genes identified may also be potential targets for therapeutics aimed at further controlling invasiveness and local destruction of BCC. |
| Databáze: | OpenAIRE |
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