Identification of Nutrient-Responsive Arabidopsis and Rapeseed MicroRNAs by Comprehensive Real-Time Polymerase Chain Reaction Profiling and Small RNA Sequencing
| Autor: | Wolf-Rüdiger Scheible, Julia Kehr, Bikram Datt Pant, Anja Buhtz, Patrick May, Dirk Walther, Przemyslaw Nuc, Magdalena Musialak-Lange |
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| Rok vydání: | 2009 |
| Předmět: |
Small RNA
Nitrogen Physiology Molecular Sequence Data Arabidopsis Plant Science Computational biology Phloem Biology Polymerase Chain Reaction Gene expression microRNA Genetics Gene Sequence Analysis RNA Gene Expression Profiling Brassica napus RNA Phosphorus biology.organism_classification Reverse transcriptase MicroRNAs Real-time polymerase chain reaction RNA Plant Research Article |
| Zdroj: | Plant Physiology |
| ISSN: | 1532-2548 |
| DOI: | 10.1104/pp.109.139139 |
| Popis: | Comprehensive expression profiles of Arabidopsis (Arabidopsis thaliana) MIRNA genes and mature microRNAs (miRs) are currently not available. We established a quantitative real-time polymerase chain reaction platform that allows rapid and sensitive quantification of 177 Arabidopsis primary miR transcripts (pri-miRs). The platform was used to detect phosphorus (P) or nitrogen (N) status-responsive pri-miR species. Several pri-miR169 species as well as pri-miR398a were found to be repressed during N limitation, whereas during P limitation, pri-miR778, pri-miR827, and pri-miR399 species were induced and pri-miR398a was repressed. The corresponding responses of the biologically active, mature miRs were confirmed using specific stem-loop reverse transcription primer quantitative polymerase chain reaction assays and small RNA sequencing. Interestingly, the latter approach also revealed high abundance of some miR star strands. Bioinformatic analysis of small RNA sequences with a modified miRDeep algorithm led to the identification of the novel P limitation-induced miR2111, which is encoded by two loci in the Arabidopsis genome. Furthermore, miR2111, miR169, a miR827-like sequence, and the abundances of several miR star strands were found to be strongly dependent on P or N status in rapeseed (Brassica napus) phloem sap, flagging them as candidate systemic signals. Taken together, these results reveal the existence of complex small RNA-based regulatory networks mediating plant adaptation to mineral nutrient availability. |
| Databáze: | OpenAIRE |
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