DNA Microarray Analysis of Estrogen Responsive Genes in Ishikawa Cells by Glabridin
| Autor: | Yong Voon Chen Phelim, Chia Yoke Yin, Visweswaran Navaratnam, Poh Su Wei Melissa |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microarray medicine.drug_class Cell Proliferative Activity Pharmacology Proteomics Bioinformatics Ishikawa cells lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology medicine lcsh:QD415-436 Gene Original Research business.industry Cancer 04 agricultural and veterinary sciences General Medicine medicine.disease 040401 food science 030104 developmental biology chemistry Estrogen business microarray Function (biology) Glabridin |
| Zdroj: | Biochemistry Insights Biochemistry Insights, Vol 10 (2017) |
| ISSN: | 1178-6264 |
| Popis: | Based on a previous study, glabridin displayed a dose-dependent increase in estrogenic activity and cell proliferative activity in Ishikawa cells. However, when treated in combination with 17β-E2, synergistic estrogenic effect was observed but without the same synergistic increase in cell proliferative effect. This study aimed to identify the estrogen and nonestrogen-regulated activities induced by glabridin and in combination with 17β-E2 in comparison with 17β-E2. The results showed that 10 µM glabridin and the combination treatment of 100 nM glabridin with 1 nM 17β-E2 regulated both the genomic and nongenomic estrogen pathways to possibly provide benefits of estrogens in cardiovascular, circulatory, and vasculature systems. Meanwhile, the combination of 100 nM glabridin with 1 nM 17β-E2 seems to be more suitable to be used as an estrogen replacement. Finally, the results of this study have added on to the present knowledge of glabridin’s function as a phytoestrogen and suggested new ideas for the usage of glabridin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|