Molecular characterization of two bacteriophages isolated from Desulfovibrio vulgaris NCIMB 8303 (Hildenborough)
| Autor: | S. Seyedirashti, J. M. Akagi, Charles E. Wood |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
viruses Restriction Mapping Genome Viral HindIII biology.organism_classification Microbiology Molecular biology Bacteriophage Endonuclease chemistry.chemical_compound Restriction enzyme Blotting Southern Microscopy Electron Restriction map chemistry DNA Viral biology.protein Bacteriophages Desulfovibrio vulgaris BamHI DNA |
| Zdroj: | Journal of general microbiology. 138(7) |
| ISSN: | 0022-1287 |
| Popis: | A preliminary endonuclease restriction map of a bacteriophage isolated from Desulfovibrio vulgaris has been established. BamHI cleaved whole phage DNA into four fragments while HindIII cut the same DNA into seven fragments. Mapping studies succeeded in linking the four BamHI fragments into two DNA segments; however, no linkage between the two segments was detected. These data imply that two phages were induced from cultures of D. vulgaris and that the two segments represented the DNA from these phages. Support for this hypothesis came from size approximation of restriction enzyme fragments, electron micrographs, and density gradients. |
| Databáze: | OpenAIRE |
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