Local trauma in human patellar tendon leads to widespread changes in the tendon gene expression
Autor: | Marc P Lorentzen, Peter Schjerling, Jacob Kildevang Jensen, Katja M. Heinemeier, Marco V. Narici, Olivier R. Seynnes, Michael Kjaer |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Cellular activity tendon injury Total RNA Total rna Messenger Gene Expression Trauma Tendons 03 medical and health sciences 0302 clinical medicine Patellar Ligament Physiology (medical) Gene expression medicine Humans human RNA Messenger 030222 orthopedics business.industry messenger RNA Tendon fat Proteins DNA medicine.disease musculoskeletal system Patellar tendon Tendon 030104 developmental biology medicine.anatomical_structure protein gene expression male metabolism patellar ligament physiology tendinitis tendon DNA Tendinopathy Wounds and Injuries Injury RNA business protein |
Zdroj: | Journal of Applied Physiology |
ISSN: | 1522-1601 |
Popis: | Low cellular activity and slow tissue turnover in human tendon may prolong resolution of tendinopathy. This may be stimulated by moderate localized traumas such as needle penetrations, but whether this results in a widespread cellular response in tendons is unknown. In an initial hypothesis-generating study, a trauma-induced tendon cell activity (increased total RNA and collagen I mRNA) was observed after repeated patellar tendon biopsies in young men. In a subsequent controlled study, 25 young men were treated with two 0.8-mm-diameter needle penetrations [ n = 13, needle-group (NG)] or one 2.1-mm-diameter needle biopsy [ n = 12, biopsy-group (BG)] in one patellar tendon. Four weeks later biopsies were taken from treated (5 mm lateral from trauma site) and contralateral tendons for analyses of RNA content (ribogreen assay), DNA content (PCR based), and gene expression for relevant target genes (Real-time RT-PCR) (NG, n = 11 and BG, n = 8). Intervention increased RNA content, and mRNA expression of collagen I and III and TGF-β1 ( P < 0.05), with biopsy treatment having greatest effect (tendency for RNA and collagen I). Results for DNA content were inconclusive, and no changes were detected in expression of insulin-like growth factor-I, connective tissue growth factor, scleraxis, decorin, fibromodulin, tenascin-C, tenomodulin, VEGFa, CD68, IL-6, MMP12, and MMP13. In conclusion, a moderate trauma to a healthy human tendon (e.g., biopsy sampling) results in a widespread upregulation of tendon cell activity and their matrix protein expression. The findings have implications for design of studies on human tendon and may provide perspectives in future treatment strategies in tendinopathy. |
Databáze: | OpenAIRE |
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