Mixed phytochemicals mediated synthesis of copper nanoparticles for anticancer and larvicidal applications
| Autor: | Sattanathan Kumar, Ambikapathi Nivetha, Theivendran Panneerselvam, Selvaraj Kunjiappan, Pavadai Parasuraman, Madasamy Sundar, Sankaranarayanan Murugesan, Sakkanan Ilango, Gopalan Rajagopal |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science (General) Nanoparticle Anticancer activity Wrightia tinctoria R.Br Q1-390 03 medical and health sciences Larvicidal activity 0302 clinical medicine Molecular docking and hemolytic assay Cytotoxicity IC50 H1-99 Multidisciplinary biology Chemistry biology.organism_classification Social sciences (General) 030104 developmental biology Phytochemical Vero cell Wrightia tinctoria Copper nanoparticles Gas chromatography GC-MS Gas chromatography–mass spectrometry 030217 neurology & neurosurgery Research Article Nuclear chemistry |
| Zdroj: | Heliyon, Vol 7, Iss 6, Pp e07360-(2021) Heliyon |
| ISSN: | 2405-8440 |
| DOI: | 10.1016/j.heliyon.2021.e07360 |
| Popis: | The synthesis of copper nanoparticles (CuNPs) using Wrightia tinctoria (Wt) R.Br extract is defined in this article as being convenient, environmentally friendly, and non-toxic. UV-visible spectrophotometry, FT-IR, XRD, particle size analyser, SEM-EDAX and TEM methods were used to describe the physicochemical properties of Wt extract mediated synthesized CuNPs (Wt-CuNPs). The Wt-CuNPs synthesized was found to be monodispersed and spherical, with an average size of 15 nm. Gas chromatography and mass spectrometry (GC-MS) research revealed that the Wt R.Br plant extract contains various phytochemical compounds. The properties of Wt-CuNPs were verified by the findings of characterization tests. Via in silico molecular docking experiments with established targets, the underlying mechanisms of cytotoxicity against breast cancer and larvicidal behaviour against Aedes aegypti of Wt-CuNPs were investigated. Interestingly, in vitro cytotoxicity studies showed 50% cell death (IC50) of Wt-CuNPs treated MCF-7 cells and Vero Cells (Kidney epithelial cells) were displayed at 119.23 μg.mL−1 and 898.75 μg.mL−1, respectively. Also, Wt-CuNPs showed least LC50 and LC90 values for larvicidal activity against A. aegypti were of 32.10 μg.mL−1 and 21.70 μg.mL−1, respectively. Furthermore, Wt-CuNPs is found to be less toxic and biocompatible in haemolytic assays. The findings clearly showed that biosynthesized Wt-CuNPs have been used as a possible anticancer and larvicidal agent, as well as being environmentally friendly. Wrightia tinctoria R.Br, GC-MS, copper nanoparticles, anticancer activity, larvicidal activity, molecular docking and hemolytic assay. |
| Databáze: | OpenAIRE |
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