A New Method for Obtaining Tumor Interstitial Fluid Applied to Cytokine Analysis of Breast Carcinoma Samples

Autor:	FELIPE TORRES DANTAS, PEDRO HENRIQUE FELIX-SILVA, HÉLIO HUMBERTO ANGOTTI-CARRARA, FRANCISCO JOSE CANDIDO DOS-REIS, MARIO TABA JUNIOR, SÉRGIO LUÍS SCOMBATTI DE-SOUZA, DANIELA BAZAN PALIOTO
Rok vydání:	2022
Předmět:	Adult Cancer Research Carcinoma Ductal Breast Interleukin-1beta Breast Neoplasms Enzyme-Linked Immunosorbent Assay Extracellular Fluid General Medicine Middle Aged Oncology Biomarkers Tumor Tumor Microenvironment Humans Female Biopsy Large-Core Needle Aged
Zdroj:	Anticancer Research. 42:1327-1332
ISSN:	1791-7530 0250-7005
Popis:	Tumor interstitial fluid (TIF), a component of the tumor microenvironment, is a valuable source of molecules and substances that help in diagnosis and prognosis of solid tumors. There is still no consensus on the optimal method for collecting TIF. Therefore, this study aimed to evaluate the effectiveness of a new method of collecting TIF in invasive ductal carcinoma (IDC) samples for cytokine interleukin 1β (IL1β) quantification.Forty women allowed the collection of TIF using absorbent paper strips during the performance of the core biopsy. The samples were stored at a temperature of -80°C and then analyzed using an enzyme-linked immunoassay.The mean values for IL1β and total protein were 11.39 mg/ml and 2.15 mg/ml, respectively.it was possible to quantify the cytokine IL1β and the total protein concentration present in the tumor tissue through TIF collection with the use of absorbent paper filters, demonstrating the effectiveness of this new method in oncology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f74fa29b424cd15891b31b54f361c3a8 https://doi.org/10.21873/anticanres.15600 Zobrazit plný text záznamu