Engineering the Dynamics of Cell Adhesion Cues in Supramolecular Hydrogels for Facile Control over Cell Encapsulation and Behavior
Autor: | Patricia Y. W. Dankers, Maartje M. C. Bastings, Simone I. S. Hendrikse, Johnick F. van Sprang, Dan Jing Wu, Freek J. M. Hoeben, Henk M. Janssen, Sergio Spaans, Maaike J. G. Schotman, Mani Diba |
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Přispěvatelé: | Biomedical Materials and Chemistry, Soft Tissue Biomech. & Tissue Eng., Protein Engineering, Biomedical Engineering, Macro-Organic Chemistry, Macromolecular and Organic Chemistry, ICMS Core |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Materials science
Supramolecular chemistry Nanotechnology Pyrimidinones macromolecular substances cell encapsulation Polyethylene Glycols Extracellular matrix Cell Adhesion Molecule Humans General Materials Science dynamic hydrogels Cell encapsulation Cell adhesion molecular exchange dynamics Fluorescent Dyes chemistry.chemical_classification Mechanical Engineering Stem Cells technology industry and agriculture Hydrogels Polymer Extracellular Matrix chemistry Mechanics of Materials supramolecular biomaterials Self-healing hydrogels Surface modification Blood Vessels synthetic extracellular matrix Fluorescence Recovery After Photobleaching |
Zdroj: | Advanced Materials, 33(37):2008111. Wiley-VCH Verlag |
ISSN: | 0935-9648 |
Popis: | The extracellular matrix (ECM) forms through hierarchical assembly of small and larger polymeric molecules into a transient, hydrogel-like fibrous network that provides mechanical support and biochemical cues to cells. Synthetic, fibrous supramolecular networks formed via non-covalent assembly of various molecules are therefore potential candidates as synthetic mimics of the natural ECM, provided that functionalization with biochemical cues is effective. Here, combinations of slow and fast exchanging molecules that self-assemble into supramolecular fibers are employed to form transient hydrogel networks with tunable dynamic behavior. Obtained results prove that modulating the ratio between these molecules dictates the extent of dynamic behavior of the hydrogels at both the molecular and the network level, which is proposed to enable effective incorporation of cell-adhesive functionalities in these materials. Excitingly, the dynamic nature of the supramolecular components in this system can be conveniently employed to formulate multicomponent supramolecular hydrogels for easy culturing and encapsulation of single cells, spheroids, and organoids. Importantly, these findings highlight the significance of molecular design and exchange dynamics for the application of supramolecular hydrogels as synthetic ECM mimics. |
Databáze: | OpenAIRE |
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