Activation and damage of endothelial cells upon hypoxia/reoxygenation. Effect of extracellular pH
| Autor: | Yu. A. Romanov, O. A. Antonova, M. V. Khachikian, S. A. Loktionova, O. N. Shustova, Mazurov Av |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Umbilical Veins
medicine.medical_specialty Endothelium Cell Vascular Cell Adhesion Molecule-1 Apoptosis Arterial Occlusive Diseases Stimulation Biology Biochemistry Statistics Nonparametric Internal medicine von Willebrand Factor medicine Extracellular Humans Cells Cultured Acidosis Analysis of Variance Caspase 3 Cell adhesion molecule Endothelial Cells General Medicine Hydrogen-Ion Concentration Hypoxia (medical) Flow Cytometry Intercellular Adhesion Molecule-1 Cell Hypoxia Oxygen medicine.anatomical_structure Endocrinology Culture Media Conditioned Endothelium Vascular medicine.symptom E-Selectin Cell Adhesion Molecules |
| Zdroj: | Biochemistry (Moscow). 74:605-612 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297909060030 |
| Popis: | Disturbances of blood flow upon vascular occlusions and spasms result in hypoxia and acidosis, while its subsequent restoration leads to reoxygenation and pH normalization (re-alkalization) in ischemic sites of the vascular bed. The effect of hypoxia/reoxygenation on activation and stimulation of apoptosis in cultured human endothelial cells was studied. The cells were subjected to hypoxia (2% O2, 5% CO2, 93% N(2)) for 24 h followed by reoxygenation (21% O2, 5% CO2, 74% N(2)) for 5 h. Reoxygenation was carried out at different pH-6.4 (preservation of acidosis after hypoxia), 7.0, and 7.4 (partial and complete re-alkalization, respectively). Hypoxia only slightly (by approximately 30%) increased the cell adhesion molecule ICAM-1 content on the cell surface, whereas reoxygenation more than doubled its expression. The reoxygenation effect depended on the medium acidity, and ICAM-1 increase was more pronounced at pH 7.0 compared to that at pH 6.4 and 7.4. Neither hypoxia nor reoxygenation induced expression of two other cell adhesion molecules, VCAM and E-selectin. Incubation of cells under hypoxic conditions but not reoxygenation stimulated secretion of von Willebrand factor and increased its concentration in the culture medium by more than 4 times. The percentage of cells containing apoptosis marker, activated caspase-3, was increased by approximately 1.5 times upon hypoxia as well as hypoxia/reoxygenation. Maximal values were achieved when reoxygenation was performed at pH 7.0. These data show that hypoxia/reoxygenation stimulate pro-inflammatory activation (ICAM-1 expression) and apoptosis (caspase-3 activation) of endothelial cells, and the extracellular pH influences both processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|