Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Autor: Ali S. Alqahtani, Anant $Other$Other Paradkar, Hayat Ouassou, Fahd A. Nasr, Ilham ElArabi, Hugo Fearnley, James Fearnley, Omar $Other$Other M. Noman, Soumaya Touzani, Badiaa Lyoussi, Shankar $Other$Other Katekhaye, Hamada Imtara, Hamza $Other$Other Mechchate
Přispěvatelé: Department of Chemistry
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Antioxidant
DPPH
medicine.medical_treatment
116 Chemical sciences
Pharmaceutical Science
Organic chemistry
High-performance liquid chromatography
Antioxidants
phenolic profile
Analytical Chemistry
chemistry.chemical_compound
0302 clinical medicine
QD241-441
Drug Discovery
Food science
Principal Component Analysis
0303 health sciences
ABTS
Bees
Anti-Bacterial Agents
propolis
Morocco
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Molecular Medicine
HONEY
Antibacterial activity
Microbial Sensitivity Tests
Gram-Positive Bacteria
Article
Cinnamic acid
CAPACITY
Middle East
03 medical and health sciences
Phenols
Picrates
Gram-Negative Bacteria
medicine
Animals
Benzothiazoles
Physical and Theoretical Chemistry
030304 developmental biology
antioxidant and antibacterial activity
Biphenyl Compounds
Polyphenols
Propolis
chemistry
Cinnamates
Polyphenol
Sulfonic Acids
HPLC
Zdroj: Molecules, Vol 26, Iss 4589, p 4589 (2021)
Molecules
Volume 26
Issue 15
Popis: The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH)
2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.
Databáze: OpenAIRE
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