An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
| Autor: | Patrícia Oliveira Meira Santos, Virgínia M G Silva, Márcia Cristina Aquino Teixeira, Washington Luis Conrado dosSantos, Geraldo G. S. Oliveira, Thiago Campanharo Bahiense, Lain Pontes-de-Carvalho, Daniela Farias Larangeira, Márcio Silva Rodrigues |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
dogs Time Factors lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:QR1-502 Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Lymphocyte Activation immunization Peripheral blood mononuclear cell lcsh:Microbiology Interferon-gamma Immune system Antigen parasitic diseases medicine Animals Hypersensitivity Delayed Dog Diseases Leishmania infantum cellular immune response Cell Proliferation Immunity Cellular biology Leishmania chagasi biology.organism_classification medicine.disease Leishmania Visceral leishmaniasis Models Animal Immunology biology.protein Leishmaniasis Visceral Antibody |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 106, Iss 2, Pp 182-189 (2011) Memórias do Instituto Oswaldo Cruz, Volume: 106, Issue: 2, Pages: 182-189, Published: MAR 2011 |
| ISSN: | 1678-8060 |
| Popis: | Domestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities. |
| Databáze: | OpenAIRE |
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