Early Pro-Inflammatory Remodeling of HDL Proteome in a Model of Diet-Induced Obesity: 2H2O-Metabolic Labeling-Based Kinetic Approach
| Autor: | Li Lin, Mirjavid Aghayev, Prabodh Sadana, Serguei Ilchenko, Takhar Kasumov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Proteomics Proteome 030204 cardiovascular system & hematology Mass Spectrometry lcsh:Chemistry Mice 0302 clinical medicine Non-alcoholic Fatty Liver Disease insulin resistance Protein Interaction Mapping Protein Interaction Maps lcsh:QH301-705.5 Spectroscopy Fatty liver General Medicine Computer Science Applications high-fat diet Factor H acute-phase proteins Disease Susceptibility Lipoproteins HDL medicine.medical_specialty diet-induced obesity Complement factor I high-density lipoprotein Diet High-Fat Complement factor B Catalysis Article Inorganic Chemistry 03 medical and health sciences Insulin resistance Internal medicine NAFLD medicine Animals Obesity Physical and Theoretical Chemistry Molecular Biology business.industry Organic Chemistry dyslipidemia nutritional and metabolic diseases medicine.disease Diet Disease Models Animal 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 inflammation Steatosis business proteome dynamics Dyslipidemia Biomarkers Lipoprotein |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 20 International Journal of Molecular Sciences, Vol 21, Iss 7472, p 7472 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21207472 |
| Popis: | Mice fed a high-fat diet for 12 weeks or longer develop hyperglycemia, insulin resistance, dyslipidemia, and fatty liver. Additionally, a high-fat diet induces inflammation that remodels and affects the anti-inflammatory and antiatherogenic property of the high-density lipoprotein (HDL). However, the precise time course of metabolic disease progression and HDL remodeling remains unclear. Short-term (four weeks) high-fat feeding (60% fat calories) was performed in wild-type male C57BL/6J mice to gain insights into the early metabolic disease processes in conjunction with a HDL proteome dynamics analysis using a heavy water metabolic labeling approach. The high-fat diet-fed mice developed hyperglycemia, impaired glucose tolerance, hypercholesterolemia without hypertriglyceridemia or hepatic steatosis. A plasma HDL proteome dynamics analysis revealed increased turnover rates (and reduced half-lives) of several acute-phase response proteins involved in innate immunity, including complement C3 (12.77 ± 0.81 vs. 9.98 ± 1.20 h, p < 0.005), complement factor B (12.71 ± 1.01 vs. 10.85 ± 1.04 h, p < 0.05), complement Factor H (19.60 ± 1.84 vs. 16.80 ± 1.58 h, p < 0.05), and complement factor I (25.25 ± 1.29 vs. 19.88 ± 1.50 h, p < 0.005). Our findings suggest that an early immune response-induced inflammatory remodeling of the plasma HDL proteome precedes the diet-induced steatosis and dyslipidemia. |
| Databáze: | OpenAIRE |
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