GL2-type homeobox gene Roc4 in rice promotes flowering time preferentially under long days by repressing Ghd7
| Autor: | Yunfei Wu, Gynheung An, Ping Jin, Yang-Seok Lee, Heebak Choi, Jinhuan Wei, Taiyong Quan, Jinmi Yoon |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors Photoperiod Flowers Plant Science Genetically modified crops 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Genes Reporter Genetics Gene Regulatory Networks Enhancer Gene Plant Proteins Regulator gene Homeodomain Proteins Reporter gene Phytochrome biology fungi food and beverages Gigantea Oryza General Medicine Plants Genetically Modified biology.organism_classification 030104 developmental biology Homeobox Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Science. 252:133-143 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2016.07.012 |
| Popis: | Under long day (LD) lengths, flowering can be delayed in rice by modulating several regulatory genes. We found activation tagging lines that showed an early flowering phenotype preferentially under LD conditions. Expression of Rice outermost cell-specific gene 4 (Roc4), encoding a homeodomain Leu-zipper class IV family protein, was significantly increased. Transcript levels of Grain number, plant height, and heading date7 (Ghd7) were significantly reduced while those of Ghd7 downstream genes were increased. However, other flowering regulators were unaffected. Whereas constitutive overexpression of Roc4 in 'Dongjin' japonica rice, which carries active Ghd7, also caused LD-preferential early flowering, its overexpression in 'Longjing27' rice, which is defective in functional Ghd7, did not produce the same result. This confirmed that Roc4 regulates flowering time mainly through Ghd7. Phytochromes and O. sativa GIGANTEA (OsGI) function upstream of Roc4. Transgenic plants showed ubiquitous expression of the β-glucuronidase reporter gene under the Roc4 promoter. Furthermore, Roc4 had transcriptional activation activity in the N-terminal region of the StAR-related lipid-transfer domain. All of these findings are evidence that Roc4 is an LD-preferential flowering enhancer that functions downstream of phytochromes and OsGI, but upstream of Ghd7. |
| Databáze: | OpenAIRE |
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