Efficient Degradation of Malathion in the Presence of Detergents Using an Engineered Organophosphorus Hydrolase Highly Expressed by Pichia pastoris without Methanol Induction
| Autor: | Dian-Sheng Xu, Yu-Lian Zhao, Chun-Xiu Li, Jian-He Xu, Xiao-Jing Luo, Yunpeng Bai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hydrolases Detergents 030106 microbiology Food Contamination Pichia Pichia pastoris Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Tap water Enzyme Stability Hydrolase Pesticides chemistry.chemical_classification Chromatography biology Pesticide residue Methanol General Chemistry Biodegradation biology.organism_classification Organophosphates Recombinant Proteins Culture Media Kinetics Biodegradation Environmental 030104 developmental biology Enzyme chemistry Malus Fermentation Biocatalysis Malathion Cucumis sativus General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:9094-9100 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b03405 |
| Popis: | The biodegradation of pesticides by organophosphorus hydrolases (OPHs) requires an efficient enzyme production technology in industry. Herein, a Pichia pastoris strain was constructed for the extracellular expression of PoOPHM9, an engineered malathion-degrading enzyme. After optimization, the maximum titer and yield of fermentation reached 50.8 kU/L and 4.1 gprotein/L after 3 days, with the highest space-time yield (STY) reported so far, 640 U L–1 h–1. PoOPHM9 displayed its high activity and stability in the presence of 0.1% (w/w) plant-derived detergent. Only 0.04 mg/mL enzyme could completely remove 0.15 mM malathion in aqueous solution within 20 min. Furthermore, 12 μmol malathion on apples and cucumbers surfaces was completely removed by 0.05 mg/mL PoOPHM9 in tap water after 35 min washing. The efficient production of the highly active PoOPHM9 has cleared a major barrier to biodegradation of pesticide residues in food industry. |
| Databáze: | OpenAIRE |
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