Multitarget Activities of Kleeb Bua Daeng, a Thai Traditional Herbal Formula, Against Alzheimer’s Disease
| Autor: | Supawadee Daodee, Pakakrong Kwankhao, Yaowared Chulikhit, Orawan Monthakantirat, Kusawadee Plekratoke, Chantha Chheng, Chantana Boonyarat, Supaporn Pitiporn, Natdanai Musigavong, Pornthip Waiwut |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Centella asiatica Antioxidant antioxidant DPPH medicine.medical_treatment Pharmaceutical Science lcsh:Medicine lcsh:RS1-441 Biology beta-amyloid aggregation Neuroprotection acetylcholinesterase inhibition Article lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Drug Discovery Survivin medicine Piper nigrum ABTS Centella Traditional medicine medicine.diagnostic_test lcsh:R Nelumbo nucifera biology.organism_classification Acetylcholinesterase 030104 developmental biology chemistry Molecular Medicine neuroprotection 030217 neurology & neurosurgery |
| Zdroj: | Pharmaceuticals, Vol 13, Iss 79, p 79 (2020) Pharmaceuticals Volume 13 Issue 5 |
| ISSN: | 1424-8247 |
| Popis: | The Kleeb Bua Daeng formula (KBD) is a Thai traditional medicine for brain health promotion. On the basis of the activities of its individual components, the KBD could have good potential for the treatment of Alzheimer&rsquo s disease (AD). Herein, we investigated the KBD as an AD treatment. The ethanol extracts of KBD and its components, i.e., Nelumbo nucifera (NN), Piper nigrum fruits (BP), and the aerial part of Centella asiatica (CA) exhibited antioxidant activity, as determined by both ABTS and DPPH assays. The Ellman&rsquo s assay revealed that the KBD, NN, and BP showed an ability to inhibit acetylcholinesterase. The thioflavin T assay indicated that the KBD, NN, BP, and CA inhibited beta-amyloid aggregation. The neuroprotection and Western blot analysis revealed that the KBD reduced H2O2-induced neuronal cell death by inhibiting the expression of pro-apoptotic factors, i.e., cleaved caspase-9 and -3, p-P65, p-JNK, and p-GSK-3&beta as well as by inducing expression of anti-apoptotic factors, i.e., MCl1, BClxl, and survivin. Furthermore, the KBD could improve scopolamine induced memory deficit in mice. Our results illustrate that the KBD with multimode action has the potential to be employed in AD treatment. Thus, the KBD could be used as an alternative novel choice for the prevention and treatment of patients with AD. |
| Databáze: | OpenAIRE |
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