Detection of astrovirus gastroenteritis in children
| Autor: | John A. Marshall, Jennifer C. Doultree, William D. Rawlinson, Huseyin Mustafa, Christopher J. McIver, Enzo A. Palombo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Serotype
viruses Sensitivity and Specificity Virus Astrovirus law.invention Immunoenzyme Techniques law Astroviridae Infections Virology Genotype Outpatients medicine Humans Adenovirus infection Polymerase chain reaction biology medicine.diagnostic_test Genetic Variation Infant biology.organism_classification medicine.disease Gastroenteritis Hospitalization Immunoassay Child Preschool Viral disease Mamastrovirus |
| Zdroj: | Journal of virological methods. 84(1) |
| ISSN: | 0166-0934 |
| Popis: | A commercial enzyme immunoassay (EIA) for the detection of astrovirus antigen was used to detect the virus during a 12-month survey of enteric pathogens in children in outpatient (n = 238) and hospital (n = 176) settings. It was found to have a 100% sensitivity and 98.6% specificity. Nineteen astrovirus isolates were detected and confirmed by northern hybridization, cell culture, and RT-PCR. The virus was detected mainly amongst outpatients although a comparison of the detection rate with that in hospitalised children did not demonstrate a statistically significant difference (p = 0.1347). In contrast, there was a strong association between hospitalization and rotavirus infection (p = 0.0371), and a strong association between infection detected in outpatients and adenovirus infection (p = 0.0193). Strains of astrovirus were sequenced, genotyped and shown to be: type 1 (n = 11), type 3 (n = 1), and type 4 (n = 7). Maximum genetic variation in type 1 isolates was 8.6% and type 4 was 7.8%. Changes did not result in amino acid substitutions. |
| Databáze: | OpenAIRE |
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