From Bugs to Bioplastics: Total (+)‐Dihydrocarvide Biosynthesis by EngineeredEscherichia coli
| Autor: | Hanan L. Messiha, Gabriel A. Ascue Avalos, Helen S. Toogood, Shirley Tait, Nigel S. Scrutton |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
engineering
Reductase 010402 general chemistry medicine.disease_cause Proof of Concept Study 01 natural sciences Biochemistry Chemical synthesis Bioplastic Lactones chemistry.chemical_compound Synthetic biology Biosynthesis Manchester Institute of Biotechnology Escherichia coli medicine Molecular Biology chemistry.chemical_classification Full Paper 010405 organic chemistry Organic Chemistry (+)-dihydrocarvide monomer Stereoisomerism Full Papers Ribosomal RNA ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology 0104 chemical sciences bioplastics Glucose Baeyer–Villiger monooxygenases Enzyme Metabolic Engineering chemistry Monoterpenes Molecular Medicine Synthetic Biology |
| Zdroj: | Ascue Avalos, G, Toogood, H, Tait, S, Messiha, H & Scrutton, N 2018, ' From bugs to bioplastics: Total (+)-dihydrocarvide biosynthesis by engineered Escherichia coli ', ChemBioChem: a European journal of chemical biology . https://doi.org/10.1002/cbic.201800606 Chembiochem |
| ISSN: | 1439-7633 1439-4227 |
| DOI: | 10.1002/cbic.201800606 |
| Popis: | The monoterpenoid lactone derivative (+)‐dihydrocarvide ((+)‐DHCD) can be polymerised to form shape‐memory polymers. Synthetic biology routes from simple, inexpensive carbon sources are an attractive, alternative route over chemical synthesis from (R)‐carvone. We have demonstrated a proof‐of‐principle in vivo approach for the complete biosynthesis of (+)‐DHCD from glucose in Escherichia coli (6.6 mg L−1). The pathway is based on the Mentha spicata route to (R)‐carvone, with the addition of an ′ene′‐reductase and Baeyer–Villiger cyclohexanone monooxygenase. Co‐expression with a limonene synthesis pathway enzyme enables complete biocatalytic production within one microbial chassis. (+)‐DHCD was successfully produced by screening multiple homologues of the pathway genes, combined with expression optimisation by selective promoter and/or ribosomal binding‐site screening. This study demonstrates the potential application of synthetic biology approaches in the development of truly sustainable and renewable bioplastic monomers. Sustainable, renewable bioplastic monomers: The monoterpenoid (+)‐ DHCD can be polymerised to form shape‐memory polymers. We have demonstrated a proof‐of‐principle approach to the complete biosynthesis of (+)‐ DHCD from glucose in E. coli, based on a modified M. spicata biosynthetic pathway. This route from a simple, inexpensive carbon source is more attractive than the alternative chemical synthesis from (R)‐carvone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text